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Microarray hybridization

samples. mRNA. cDNA. DNA microarray. Microarray hybridization. Usually comparative Ratio between two samples Examples Tumor vs. normal tissue Drug treatment vs. no treatment Embryo vs. adult. 2. Spotting DNA on the chip. Types of printing pins. Apply small droplets on contact.

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Data Analysis DNA Microarrays

Data Analysis DNA Microarrays. Dr. Rebecca Fry. Raw Data File. ID Cy3 Spot Mean Cy3 Background Cy5 mean Cy5 Background. Data Analysis. Step 1-Subtract Background Intensity from each spot. Generate Excel Formula. Paste into all cells. Data Analysis.

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Alexa594

Cy5 labelled sample. Alexa labelled sample. Log 2 (signal). Log 2 (signal). 4. 6. 4. 6. 6. 4. 7. 7. 7. 5. 5. 5. Array:. Array:. 2. 2. 2. 3. 3. 3. 1. 1. 1. Alexa594. Cy5. Cy3. Alexa594. Cy5. Cy3. Cy3 labelled sample. Log 2 (signal). Array:. Alexa594. Cy5. Cy3.

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Microarray hybridization

Data Analysis DNA Microarrays

Alexa594