'Eco ri' diaporamas de présentation

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Chapter 2

Chapter 2. Enzymes for Genetic Engineering. 2.1 Restriction endonucleases 2.2 DNA ligase 2.3 DNA polymerase 2.4 Other Enzymes 2.5 probe labeling techniques. ??? ???????? ???????? 1. ?????????????????????????????? 2 ???????????????????????????????????? ????? 1 ????????? 2 ????

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Chapter 4 Molecular Cloning Methods

Chapter 4 Molecular Cloning Methods. Jay D. Hunt, Ph.D. Department of Biochemistry and Molecular Biology CSRB 4D1 568-4734 jhunt@lsuhsc.edu. I. Restriction Endonucleases. Restriction endonucleases

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Introduction to Cloning and Recombinant DNA Technology

Introduction to Cloning and Recombinant DNA Technology. David Bedwell, Ph.D. Department of Microbiology Office telephone: 934-6593 Email: dbedwell@uab.edu The Powerpoint slides for this lecture are available for download at: http://www.microbio.uab.edu/bedwell/index4.html

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Introduction to Cloning and Recombinant DNA Technology

Introduction to Cloning and Recombinant DNA Technology. David Bedwell, Ph.D. Department of Microbiology Office telephone: 934-6593 Email: dbedwell@uab.edu The Powerpoint slides for this lecture are available for download at: http://www.microbio.uab.edu/bedwell/index4.html

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Addressable Bacterial Conjugation

Addressable Bacterial Conjugation. UC Berkeley iGEM 2006. Bryan Hernandez Matt Fleming Kaitlin A. Davis Jennifer Lu Samantha Liang Daniel Kluesing Will Bosworth. Advisors: Professors Adam Arkin and Jay Keasling GSIs: Chris Anderson and John Dueber. Project Goal.

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Cloning of the genes that code for three major subunits of Escherichia coli polymerase III

Cloning of the genes that code for three major subunits of Escherichia coli polymerase III. Chengxi Shi Molecular Biotechnology and Bioinformatics Uppsala University winter,2005. About Research Training. Period: November to December

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Biotechnology: Tools and Techniques of the Trade

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100 copy

Fig. S1. A. TG mice. NTG. Plasmid control. 10 copy. 100 copy. 1 copy. NTG. NTG. 36. 41. 74. 76. 78. 90. 97. 98. 105. Enzyme ? Eco-RI. I2020T LRRK2: 3,404 bp. B. 10K. Bgl II. Eco RI. 8. 41. 74. B6. 41. 74. B6. 6. 5. 4. 3. 2.5. 2. 1.5. 1.

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X: Xba I

10. 12. 14. 18. 20. X: Xba I. E: Eco RI. H: Hind III. 3. 4. 5. 6. X: Xba I. E: Eco RI. H: Hind III.

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Bam HI

Undigested DNA. Hae III. Cla I. Eco RI. Bam HI. Hin dIII. Ladder.

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P cry3Aa

Plasmids. Vector. Fusion genes. Eco RI. Xba I. Bgl II. Pst I. P cry3Aa. mbg. gfp. pMB163. Eco RI. Xba I. Bgl II. Pst I. P cry3Aa. mbgn. gfp. pMB164. Eco RI. Xba I. Bgl II. Pst I. P cry3Aa. mbgc. gfp. pMB165. Eco RI. Xba I. Bgl II. Pst I. P cry3Aa. lysM 1. gfp.

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10h

(a). Tgt1 (9,446bp). Xba I. Xba I. Eco RI. Eco RI. PBS. PPT. LTR. LTR. 2180bp. 2180bp. AGCAG. AGCAG. ORF1 (488aa). ORF2 (1152aa). intron. f3?5?h2 Tgt1. exon1. exon2. (b). 1. 2. 1. 2. (kbp). 10-. 6-. 4-. 3-. 2-. 1-. 1h. 10h. Exposure time. Fig. S5.

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