'Fluorescence resonance energy transfer' diaporamas de présentation

Critique (everyone) Monitoring dynamic protein interactions with photoquenching

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Resonance Energy Transfer

Resonance Energy Transfer. Non-Radiative Energy Transfer driven by dipole-dipole coupling. Fluorescence Resonance Energy Transfer Surface Energy Transfer. Multiplicity : A property of a system due to the spin, or angular

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FLUORESCENT LABELING OF BIOMOLECULES WITH ORGANIC PROBES

FLUORESCENT LABELING OF BIOMOLECULES WITH ORGANIC PROBES. Soundarya Vaithianathan Graduate Student Department of Medicinal Chemistry, School of Pharmacy, VCU. email: vaithianaths@mymail.vcu.edu. February 12 th , 2010. WHAT ARE BIOMOLECULES ?.

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Fluorescent Techniques in PCR

Fluorescent Techniques in PCR. Cao jian ping. PCR. Amplification of specific gene or gene fragments Process of PCR 1. Denaturation 2. Annealing 3. Extension. Traditional way to assay the result of PCR:

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Biophysics 101: Genomics & Computational Biology Section 8: Protein Structure

Biophysics 101: Genomics & Computational Biology Section 8: Protein Structure. Faisal Reza Nov. 11 th , 2003 B101.pdb from PS5 shown at left with: animated ball and stick model, colored CPK H-bonds on, colored green van der Waals radii on, also colored CPK

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Green Fluorescent Protein

Green Fluorescent Protein. Barzilai Sharon Elissar Naama. Wild Type GFP. GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. GFP is a protein which exhibits bright green fluorescence when exposed to blue light. The protein composed of 238 amino

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Class 5 Immuno -assay with magnetic nanoparticle tags

Class 5 Immuno -assay with magnetic nanoparticle tags Gaster et al Nature Medicine 15:1327 (2009) Basic idea Giant magneto resistance (GMR) Sensor characterization. Sandwich immunoassay with superparamagnetic particle tags and GMR detection of magnetic labels.

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Crystallization and Dimer Exchange of the Protein Superoxide Dismutase

Crystallization and Dimer Exchange of the Protein Superoxide Dismutase . Emily Clark Dr. Joe Beckman Department of Biochemistry/ Biophysics Oregon State University. Amyotrophic Lateral Sclerosis Louis Gehrig Disease. Fatal neurodegenerative disease targeting motorneurons

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Nanosensors

Nanosensors. Hyunkyung Bae Byungmook Kim. Why is it needed?. How to make?. What to do?. Nanosensors. Big Issues in Biology. Google image. Properties of Nanosensors. 1. Magnetic Property. 2 . Optical property. Google image. Magnetic properties. Superparamagnetism.

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Methods: Fluorescence

Methods: Fluorescence. Biochemistry 4000 Dr. Ute Kothe. Remember: Absorbance. Absorbance of monochromatic light reduces the intensity (I) Measured relatively to original intensity (I 0 )

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Molecular chaperones involved in degradation and other processes (I)

Molecular chaperones involved in degradation and other processes (I). 21-1. Chaperones involved in degradation - AAA ATPases: background - ClpA functional mechanism - katanin functional mechanism - archaeal PAN , VAT. AAA ATPases: functions. 21-2.

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Biophysical Approaches to Drug Design

Biophysical Approaches to Drug Design. Overall Organization of the Topic. Approaches Useful in Drug Design Fluorescence Spectroscopy - Principle of Fluorescence - Factors governing fluorescence intensity - Fluorescent groups - Sensitivity of Fluorescence to Environment

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Quenching

Quenching. Non- radiative energy transfer from excited species to other molecules. Quantum Yield and Quenching. Show that quantum yield in the presence of a quencher is:. F A,p = k A n S0 V. F F,p = k F n S1 V. Dynamic Quenching/ Collisional Quenching

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Flow Cytometric Analysis of FRET to Study the Interaction Between CFP- and YFP-Tagged Proteins

Flow Cytometric Analysis of FRET to Study the Interaction Between CFP- and YFP-Tagged Proteins. David Stepensky. Classical pathway of major histocompatibility complex (MHC) class I antigen processing, loading, and presentation. Groothuis et al, Immunol Rev 2005. Objectives.

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Resonant Enhancement and Dissipation in Nonequilibrium van der Waals Forces

Resonant Enhancement and Dissipation in Nonequilibrium van der Waals Forces. Adam E. Cohen (Stanford) Shaul Mukamel (UC Irvine). 3 + 3 = 6. Gauss : if. and f is insensible between macroscopic objects, then. van der Waals :. a ~ index of refraction (empirically). McLachlan :. T = 0:.

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Fluorescent Proteins By James Dicarlo and Ingrid Spielman August 12, 2008

Fluorescent Proteins By James Dicarlo and Ingrid Spielman August 12, 2008. GFP chromophore. Aequorea victoria. Tons of different Fluorescent Proteins . Shape. Discosoma is a coral. Conjugated Double Bonds. p-hydroxybenzylidene-imidazolidone. Stokes Shift. Conjugated double bonds:

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