'Fusion protein' diaporamas de présentation

August 22, 2011 Biochemistry 201 D. Worthylake, 7152MEB, x5176

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Second and third generation therapeutic and diagnostic proteins Directed evolution of new proteins

Second and third generation therapeutic and diagnostic proteins Directed evolution of new proteins Connect phenotype (typically a binding) to genotype (typically DNA) Displayer size Number Membrane display (Rice paper) High Low Yeast surface display E. coli surface display

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Second and third generation therapeutic and diagnostic proteins Directed evolution of new proteins

Class 24 last updated 11/30/11 1:00 AM. Second and third generation therapeutic and diagnostic proteins Directed evolution of new proteins Connect phenotype (typically a binding) to genotype (typically DNA) Displayer Size Number Membrane display (Rice paper) High Low

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Protein Purification Involving a Unique Auto-Cleavage Feature of a Repeated EAAAK Peptide

Protein Purification Involving a Unique Auto-Cleavage Feature of a Repeated EAAAK Peptide. Interdisciplinary Research of Enzyme Technology. Protein Production. Structure & Catalytic Mechanism. Bio-conversion. Enzyme Research. Bio-medical application.

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Protein engineering and recombinant protein expression

Prof. FATCHIYAH, M.Kes.PhD http://fatchiyah.lecture.ub.ac.id. Protein engineering and recombinant protein expression. Outline. Why bother with recombinant fusion protein or protein engineering? Principle in recombinant p rotein expression

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GENETIC MUTATIONS

GENETIC MUTATIONS. Section 5.6 Pg. 259. Mutation : A change in the DNA sequence that is inherited as the DNA is transmitted through cell division. Categorising mutations. By effect on structure By consequences on resulting proteins. 1. Mutations by effect on structure. Point mutations

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Expression of clones genes

Expression of clones genes. a.o. Primrose & Twyman, 7th edition, pp.81-93 Primrose, Twyman & Old, 6th edition, pp.70-83. IPTG-induced expression of RP4 proteins : (A) DNA primase (118 and 80 kDa proteins) ; (B) 16.5 and

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Protein domains vs. structure domains - an example.

Protein domains vs. structure domains - an example. Genome of the week. Nanoarchaeum equitans - archaea Hyperthermophile Diverged early in evolution from other archaea New kingdom of archaea? Obligate symbiont with Ignicoccus Smallest completely sequenced genome <500kB

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C105A/C205A Uland Lau, Pardeep Singh, Joe Argus 4/17/12

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PDGF receptor ? and leukemia

PDGF receptor ? and leukemia. Caleb Parker. Overview. What is PDGFRB? What role does PDGFRB play in the cell? What is CMML? What is Tel/PDGFRB? What is the connection between CMML and Tel/PDGFRB? What are the potential therapies for CMML?. What is PDGF?. Platelet-derived growth factor

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Affinity Chromatography

Affinity Chromatography. What is it used for?. Monoclonal and polyclonal antibodies Fusion proteins Enzymes DNA-binding proteins . . . . . . ANY protein where we have a binding partner!!. Steps of Affinity chromatography. 1. Equilibration.

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Mumps (parotitis)

Mumps (parotitis). Dr. Mohammed Arif Associate professor Consultant virologist Head of the virology unit. Mumps (parotitis). Inflammation of the salivary glands. Mainly the parotid glands are affected. There are three pairs of salivary glands.

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??????. ??????. Plasmid DNA miniprep . PCR R. E. digestion Gel purification, Ligation, Transformation Preparation of competent cells. Gene Plasmid b.p . ORF E.coli strain. Recombinant DNA Techniques (DNA Cloning). GST-LEU2 SDS-PAGE Coomasie Blue ( Green Angel) Staining

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Why synthetic Biology?

Why synthetic Biology?. Reverse Engineering vs. Forward engineering: Synthetic replicas of natural genetic circuits. 1) Why synthetic Biology?. Reverse Engineering vs. Forward engineering: Synthetic replicas of natural genetic circuits Test sufficiency of design of producing behavior

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Phage Display and its Applications

Phage Display and its Applications. Matt Brown Human Genetics Dr. Nancy Bachman. Used for cloning foreign genes among other applications Proteins and peptides are fused to the Capsid (surface) of the phage The combination of the phage and peptide is known as a Fusion Protein. Basics.

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Other affinity proteins

Other affinity proteins. Other affinity proteins / ligands. Single-chain variable fragment. Exercise 1. Bibliography search - Describe the structure of one of the following affinity protein (ScFv, diabody, affibody, avimer).

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Targeting cancer cells by using an photoimmunosensitizer - 4D5scFv-KillerRed Kamil Tomaszewski

Targeting cancer cells by using an photoimmunosensitizer - 4D5scFv-KillerRed Kamil Tomaszewski.

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Investigation of Arabidopsis thaliana proteins AtSNX2a and AtSNX1

Investigation of Arabidopsis thaliana proteins AtSNX2a and AtSNX1 Lucas Greder and Diane Bassham, National Science Foundation-Research Experience for Undergraduates, Iowa State University, Ames, Iowa.

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Genetically Engineered Materials Science & Engineering Center

Genetically Engineered Materials Science & Engineering Center Mehment Sarikaya, University of Washington, DMR 0520567. Protein-Driven Synthesis of Transition Metal-Doped ZnS Immuno-Quantum Dots.

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