Ipg strip - PowerPoint PPT Presentation

MALDI- TOF

BIOCHEMISTRY

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Proteomics Day 3 Tech Talk

Proteomics Day 3 Tech Talk. Activities. 1. Prepare IPG strip for second gel electrophoresis 2. Run IPG strip in second dimension SDS-PAGE electrophoresis gel. 3. Stain with Coomassie Blue. Equilibration of IPG strip.

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202 views • 7 slides

Proteomics Day 2 Tech Talk

Proteomics Day 2 Tech Talk. Activities. 1. Run 1 dimension SDS-PAGE gel of proteins isolated on day one and stain with Coomassie Blue

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178 views • 6 slides

Technical overview IPG and 2D

Technical overview IPG and 2D. UVM Proteomics Outreach Module. Rehydrating the IPG strip Loaded sample Applied IPG strip Overlay with mineral oil-prevents Drying of the strip Crystallization of the urea Uptake of O2 and CO2 50 volts overnight. Next Day….

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215 views • 5 slides

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