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Electrophoresis

Electrophoresis

Electrophoresis. Separation, analytical or preparative, of charged molecules by migration through a matrix due to application of an electric field, with net movement towards electrode of opposite charge. General Principle.

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795 views • 42 slides
Electrophoresis and 2D Gel Analysis

Electrophoresis and 2D Gel Analysis

Electrophoresis and 2D Gel Analysis. What is Electrophoresis ? The migration of a charged particle (protein) through a separation matrix toward the opposite charged electrode. Anion. Cation. Anode. Cathode. Types of Electrophoresis. 1D Gel Electrophoresis- DNA, RNA, and Protein

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723 views • 36 slides
A meer Effat Elfarash

A meer Effat Elfarash

Different applications of protein electrophorasis. A meer Effat Elfarash. Dept. of Genetics Fac. of Agriculture, Assiut Univ. aelfarash@aun.edu.eg. Principle. Proteins move in the electric field. Their relative speed depends on the charge, size, and shape of the protein.

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283 views • 28 slides
Targeting and assembly of proteins destined for chloroplasts and mitochondria

Targeting and assembly of proteins destined for chloroplasts and mitochondria

Targeting and assembly of proteins destined for chloroplasts and mitochondria. How are proteins targeted to chloroplasts and mitochondria from the cytoplasm? How do they get through the membranes?. Two types of cytosolic ribosomes: free and membrane-bound.

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420 views • 23 slides
Targeting and assembly of proteins destined for chloroplasts and mitochondria

Targeting and assembly of proteins destined for chloroplasts and mitochondria

Targeting and assembly of proteins destined for chloroplasts and mitochondria. How are proteins targeted to chloroplasts and mitochondria from the cytoplasm? How do they get through the membranes?. Two types of cytosolic ribosomes: free and membrane-bound.

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366 views • 23 slides
Buffers

Buffers

Buffers. pH measurement. Voltage ? log [H + ] inside /[H + ] outside. Buffers. Buffering capacity Phosphate, carbonate, Tris buffers Good buffers. Electrophoresis. Bible: Gel Electrophoresis of Proteins: Practical Approach, Hammes and Rickwood, IRL Press Principles

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802 views • 15 slides
How is the energy of Oxidation Preserved for the synthesis of ATP?

How is the energy of Oxidation Preserved for the synthesis of ATP?

How is the energy of Oxidation Preserved for the synthesis of ATP?. ANS: Electron transfer to oxygen is accompanied by the formation of a high energy proton gradient. The Gradient arises by having protons pumped from the matrix side of the mitochondria to

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285 views • 15 slides
Protein Identification using 2D LC/MS/MS based on pH Gradient and Reverse Phase Separation

Protein Identification using 2D LC/MS/MS based on pH Gradient and Reverse Phase Separation

Protein Identification using 2D LC/MS/MS based on pH Gradient and Reverse Phase Separation. Column Technology Inc.,. Part I. Comparison of salt and pH step gradient. pH Based 2D-LC-MS/MS: pH 3.0-8.0, Buffer (5-10mM) v.s. Salt Based 2D-LC-MS/MS: salt 0-2000 mM, pH ~2.5.

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270 views • 13 slides
How to set up a CIEF experiment

How to set up a CIEF experiment

How to set up a CIEF experiment. This presentation is intended for new capillary electrophoresis users who are part of the Biomolecular Interaction Technologies Center (BITC). Background.

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173 views • 3 slides

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