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Spectrophotometric Determination of the pKa of BTB

Spectrophotometric Determination of the pKa of BTB. Lab 7. Outline. Purpose Bromothymol Blue Indicators Reaction Equilibrium Expression pK a Determination Procedure Safety Concerns Waste Next Lab Reminder. Purpose.

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555 views • 16 slides

Buffers

Clinical Analytical Chemistry CLS 231. Buffers. Lecture 3 Lecturer: Amal Abu Mostafa. Session Objectives:. Buffer solution Composition of a Buffer Examples of buffers How a Buffer Works Preparing Buffers with a Desired pH Henderson Hasselbalch equation

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548 views • 15 slides

Determining the k a of an acid-base indicator

Determining the k a of an acid-base indicator. Experiment 3. Indicator. HIn + H 2 O ? In - + H 3 O + Red blue Ka = [H 3 O + ] [In - ]/[HIn] [H 3 O + ] = Ka [HIn]/[In - ] (-log [H 3 O + ]) = (-log Ka) + (log [In - ]/[HIn]) pH = pKa + log [In - ]/[HIn]. Determining Ka.

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257 views • 14 slides

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Spectrophotometric Determination of the pKa of BTB

Buffers

Determining the k a of an acid-base indicator