Phenol chloroform - PowerPoint PPT Presentation

Rec. DNA Lab Wednesday, Jan 24, 2007

Rec. DNA Lab Wednesday, Jan 24, 2007. Isolation of Chromosomal DNA from Photobacterium leognathi. Grow culture Lyse cells Remove unwanted cellular components Precipitate DNA and dissolve in appropriate buffer. Some important reagents: Lysozyme EDTA SDS Phenol/Chloroform Proteinase K

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Cell ( E. coli ) down (maximum speed for 30 seconds)

Cell ( E. coli ) down (maximum speed for 30 seconds). supernatant (medium) bacterial pellet. Alkaline lysis solution I (100 µl)-vigorously vortexing. Alkaline lysis solution II (200 µl)-inverting five times

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