'Plasmid dna' diaporamas de présentation

• History of cell-substrate DNA in biological products • Methods used to quantify DNA • Perceived safety issues associated with DNA • Review of assays and published data on the biological activity of DNA • Development of quantitative assays to assess risk

pGLO ™ & GFP. Uses of Green Fluorescent Protein. Uses of GFP. GFP is a visual marker Study of biological processes (example: synthesis of proteins) Localization and regulation of gene expression Cell movement Cell fate during development Formation of different organs

Image Gallery. NanoDynamics Systems Lab Iowa State University. Yeast on Agar. Square Gratings: 20nm Height. Probe Loss: ‘Dead Zones’ of AFM Imaging. Dynamic Gain Adjustment for dead zones. Improvement in probe loss without sacrificing on sample behavior. Graphite Surface.

Restriction Enzyme Digestion . Zelha Nil Nov-09. Today’s Laboratory Objectives. Results of gDNA experiment: the concentration, purity, and integrity of genomic DNA Digest genomic DNA and plasmids . DNA quantification.

Announcements. Lab reports (X-linked cross) due today - start of lecture 2. Pick up lab overview 12 - read and answer pre-lab questions, due at start of lab (instead of quiz!). 3. On 3x5 card - write 1-2 specific topics you would like “reviewed” before the exam or questions you have

Useful hints and tips for cloning, PCR and site-directed mutagenesis. Kirsten Jensen Division of Molecular Biosciences. Overview. Cloning (of PCR products) Polymerase chain reaction (PCR) Site-directed mutagenesis. MCS. Cloning of a region of interest into a plasmid. Plasmid.

Laboratory: Bacterial Transformation. Introduction of plasmid DNA into E. coli. This laboratory is. The first part in a series of 3 experiments: Plasmid Transformation Plasmid Isolation Plasmid Mapping. Transformation. A process of plasmid DNA uptake

cDNA microarrays on glass slides. An overview of the Brown-De Risi- Iyer technology, based on the 2000 CSH Microarray Course notes, Nature Genetics Supp, Jan 1999,

Detection and Identification of Microorganisms. Chapter 12. Applications of Molecular Based Testing in Clinical Microbiology. Rapid or high-throughput identification of microorganisms Those that are difficult or time-consuming to isolate e.g., Mycobacteria Hazardous organisms

Exploring the Use of Plasmids in DNA Microarray Technology B. Daniel Pierce and A. Malcolm Campbell Biology Department, Davidson College, Davidson, NC 28035. 1:1 Probe Ratio Results . Abstract. Chip Design and Sample Scans.

Recombinant DNA Techniques Laboratory Bi 431/531. Introduction/Syllabus Class Overview Bioluminescent bacteria Micropipetting – Exercise I, part I Aseptic techniques – Exercise I, part II Environmental Isolates – Exercise III Start liquid cultures for Wednesday. Introduction/Syllabus.

pAmylase Restriction Digestion (Lab 8b). “DNA is the Flash but Proteins are the Cash of Biotech ”. ~40 bp. Ellyn Daugherty SMBCP, San Mateo CA www.BiotechEd.com www.SMBiotech.com www.emcp.com/biotech www.sargentwelch.com/biotech. The Amylase Project Model of rDNA/protein Business.

Recombinant DNA Technology and Genomics. Chapter Contents. 3.1 Introduction to Recombinant DNA Technology and DNA Cloning 3.2 What Makes a Good Vector? 3.3 How Do You Identify and Clone a Gene of Interest?

Biotechnology . The use of living organisms to solve industrial tasks. . Restriction enzymes. A.K.A restriction endonuclease Enzymes which cuts DNA along sugar- phosphate backbone “Cuts” DNA at Restriction Sites. Restriction site.

The topology of nucleic acids. Relevant aspects: The number of bases per turn is not fixed, but can be adjusted depending on the circumstances.

Species-specific neuronal circuits directed by neurotrophic factor control of transcriptional programmes. > novel neural circuits arise during evolution to encode unique behaviors among different animal species. Chick. Mouse. > hopping gait = sautiller > feathers.

DNA fingerprinting. Look at the DNA of the mother, father and child Could these parents produce this child?. DNA fingerprinting is used to determine paternity. DNA fingerprinting. 2 methods Southern Blot and Restriction fragment length polymorphism PCR and VNTRs. Different types of DNA.

FTCE SAE Biology Preparation Course. Instructor Valerie Ruwe vruwe@browardschools.com. Session Norms. No side bars Work on assigned materials only Keep phone on vibrate only If a call must be taken please leave the room to do so. Session Agenda. Session I: Pre-Test, Competencies 1 & 2

Coupling teaching and research. Incorporating a research project into a required course. Fall 2003 Centenary College Fall: Sophomore Cell Biology (2 lectures, 3 labs) Spring: Junior Genetics (1 lecture, 2-3 labs) and junior/senior course (e.g., Advanced Cell Bio, Cancer Bio). Summer:

Biotechnology and Bacterial Transformation. ABE Lab sequence. Creating a Recombinant Plasmid. Restriction and Ligation. We used restriction enzymes to cut the two plasmids at the certain points