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Supplementary Figure S1

Supplementary Figure S1. a. b. Asyn. Noco. Asyn. Noco. DMSO. DMSO. XAV939. XAV939. -. +. -. +. PPase. TNKS1. 150. TNKS1. 150. IP ( α TNKS1). PAR. IP ( α TNKS1). PAR. 150. 150. PTTG1. Cyclin B1. Actin. Actin. Supplementary Figure S2. shLuc. shPlk1. pHA. pHA-Plk1.

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Isolation of Centrosome-Associated Polo-Box Domain-Binding Proteins

Make Primers. Cloning procedures. GST Polo-Box Domain Pull down assay. Western/Immuno Blotting. P. p-T78 peptide. : PLH S T 78 A I ( in vitro by Plk ). Nhe1(dead) AscI HA tag PmeI EcoRV XhoI NotI. 491 WT, AM, Input. 485 WT, AM, Input. 497 WT, AM, Input.

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