'Rna quality' diaporamas de présentation

2100 Bioanalyzer

2.35k views • 76 slides

Abdelilah Soussi Gounni, Ph.D Associate Professor Manitoba Research Chair

Quantification of mRNA using real-time PCR. Abdelilah Soussi Gounni, Ph.D Associate Professor Manitoba Research Chair Department of Immunology University of Manitoba. Objectives Introduction to qPCR Chemistries used in qPCR Different platforms : pro and con

862 views • 68 slides

TISSUE PROCUREMENT AT CANCER CENTERS

TISSUE PROCUREMENT AT CANCER CENTERS. Carl Morrison M.D., D.V.M. Roswell Park Cancer Institute. 1) FUNCTIONAL REQUIREMENTS: What are the operational standards that should be acceptable for TP at any Cancer Center???? 2) INFRASTRUCTURE ISSUES: Costs, administration, Department priorities????

713 views • 55 slides

Chris J. Sullivan, Ph.D. Department of Biological Sciences

Microarrays: Gene Expression Data to Biological Insight. Chris J. Sullivan, Ph.D. Department of Biological Sciences. The Scientific Method. Observation. Before a testable hypothesis and experiments comes?. Microarrays - Global Gene Expression Hypothesis Generation.

634 views • 48 slides

Microarray Basics

Microarray Basics. Part 1: Choosing a platform, setting up, data preprocessing. Experimental design. What type of microarray What overall design strategy How many replicates. Type of Microarray. One colour. Two colour. Short oligo. cDNA. Long oligo. Genome wide. Custom.

600 views • 47 slides

High throughput expression analysis using RNA sequencing ( RNAseq )

High throughput expression analysis using RNA sequencing ( RNAseq ). June 18, 2019. Lecture objectives. Theory and practice of RNA sequencing (RNA- seq ) analysis Rationale for sequencing RNA Challenges specific to RNA- seq Types of libraries for RNA- seq Sequencing coverage (depth)

1.15k views • 41 slides

Stat 2411 Statistical Methods

Stat 2411 Statistical Methods. Chapter 2: Summarizing data. Summarizing Data. Data are collected to answer some questions. The analysis of the data includes thinking and statistical methods. Example: 8 lb test Fishing Line Question: Which type(s) of line are strongest?.

432 views • 29 slides

Identification and functional characterisation of molecular risk factors in acute leukemias

Lab of the study group: Isolation of mononuclear cells. Cryopreservation. RNA Preparation. RNA Preparation. Initial ALL (ALL-BFM trial). Relapsed ALL (ALL-REZ BFM trial). Classification. Retrospective Study:. Event-free Survival ( ? 3 years). 1. Relapse. Event-free Survival

93 views • 1 slides