'Serine proteases' diaporamas de présentation

THE PROTEASES

THE PROTEASES. -. +. 3 big problems for the kinetics: H 2 O is a bad nucleophile NH is a bad leaving group loss of resonance during reaction No problem for thermodynamics ( D G << 0 ). D G < 0. H 2 O:. THE PROTEASES. H 2 O.

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Enzyme Mechanisms and Regulation

Enzyme Mechanisms and Regulation. Andy Howard Introductory Biochemistry, Fall 2010 Wednesday 22 September 2010. How do enzymes reduce activation energies?. We want to understand what is really happening chemically when an enzyme does its job.

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Catalytic Strategies

Catalytic Strategies. Basic Catalytic Principles. What is meant by the binding energy as it relates to enzyme substrate interactions? free energy released by formation of weak interactions between enzyme and substrate How is binding energy used in catalysis? establishes substrate specificity

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What is enzyme catalysis?

What is enzyme catalysis?. A catalyst is a substance that accelerates a chemical reaction without itself undergoing any net change. How do enzymes work?. Free energy. Thermodynamics of catalysis. D G = D H - T D S. D G = Gibbs Free energy D H = Change in heat (energy)

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Enzymes V: Specific Mechanisms; Regulation

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Classifying the protein universe

Synapse-Associated Protein 97 Classifying the protein universe Wu et al, 2002. EMBO J 19:5740-5751 Domain Analysis and Protein Families Introduction What are protein families? Protein families Description & Definition Motifs and Profiles The modular architecture of proteins

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Disulfide Bonds

Disulfide Bonds. Two cyteines in close proximity will form a covalent bond Disulfide bond, disulfide bridge, or dicysteine bond. Significantly stabilizes tertiary structure. Determining Protein Structure. There are O(100,000) distinct proteins in the human proteome.

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Enzymes: mechanisms & regulation

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Lecture 17

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Enzymes

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Modeling Escherichia coli signal peptidase complex with bound substrate: Determinants in mature peptide influencing sig

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Chapter 15 Enzymatic Catalysis

Chapter 15 Enzymatic Catalysis. Read Chapter 15: Sections 1 & 3 (pp. 496-507, 515-528). Active site of a serine protease. Types of Catalysis. Types of catalytic mechanisms that enzymes employ:. Acid-base catalysis Covalent catalysis Metal ion catalysis Electrostatic catalysis

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Mining Patterns in Protein Structures Algorithms and Applications

MotifSpace. Mining Patterns in Protein Structures Algorithms and Applications. Wei Wang UNC Chapel Hill weiwang@cs.unc.edu. Proteins Are the Machinery of Life. Protein Structure Initiative. Function. Spatial motifs. Protein Data Bank. Serine protease. Papain-like Cysteine protease .

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Enzyme Catalysis-Serine Proteases

Enzyme Catalysis-Serine Proteases. Concepts to be learned Activation Energy Transition State Example: Proteases Requirements for proteolysis Families of proteases Protein Folds used by proteases for catalysis. Catalysis.

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Homology modelling of serine proteases

Homology modelling of serine proteases. Industrial examples. Roland J. Siezen. Industrial enzymes. Enzyme Source Application Market(US$m) alkaline proteases Bacillus detergents 150 neutral proteases Bacillus, Aspergillus baking, brewing 70

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Trypsin and Chymotrypsin

Trypsin and Chymotrypsin. Protein digestion. Breaking down protein into absorbable amino acids and some di- and tri-peptides. Parts of a polypeptide.

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S2 L6 Pharmaceutical uses of enzymes

S2 L6 Pharmaceutical uses of enzymes. Anna Drew. Enzymes. catalyse (speed up) chemical reactions involved in most processes in a biological cell proteins specific activity affected by other molecules (inhibitors) temperature chemical environment (pH) concentration of substrate

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The Blood Clotting Cascade

The Blood Clotting Cascade. Manfred Maitz FZ-Rossendorf www.manfred.maitz-online.de. Thrombin. Fibrin polymers. Fibrin monomers. Fibrinogen. The Clotting Cascade. Intrinsic Pathway. Extrinsic Pathway. Surface Contact Collagen FXII activator. Tissue/Cell Defect. F XII. F XIIa.

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Enzyme Mechanisms: Serine Proteases

Enzyme Mechanisms: Serine Proteases. C483 Spring 2013. Questions. 1 . The substrate specificity of serine proteases is primarily due to A ) a specificity pocket in the protein. B ) the positions of specific side chains of serine, histidine , and aspartate.

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