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Genome sequencing and assembling

Genome sequencing and assembling

Genome sequencing and assembling. Chitta Baral. Basic ideas and limitations. Current lab techniques can sequence small (say 700 base pairs) DNA pieces. Use restriction enzymes to cut DNA pieces Sort pieces of different sizes using gel electrophoresis and use the sorting to read them

By anastacia
(233 views)

Linkage Disequilibrium-Based Single Individual Genotyping from Low-Coverage Short Sequencing Reads

Linkage Disequilibrium-Based Single Individual Genotyping from Low-Coverage Short Sequencing Reads

Linkage Disequilibrium-Based Single Individual Genotyping from Low-Coverage Short Sequencing Reads. Justin Kennedy 1 Joint work with Sanjiv Dinakar 1 , Yozen Hernandez 2 , Ion Mandoiu 1 , and Yufeng Wu 1 1 CSE Department, University of Connecticut

By isabel
(342 views)

C A M E R A A Metagenomics Resource for Marine Microbial Ecology

C A M E R A A Metagenomics Resource for Marine Microbial Ecology

C A M E R A A Metagenomics Resource for Marine Microbial Ecology. July 27, 2007 Paul Gilna UCSD/Calit2 Saul A. Kravitz J. Craig Venter Institute. Acknowledgements. UCSD/Calit2 Larry Smarr, PI; Paul Gilna, Executive Director Phil Papadopoulos, Technical Lead Weizhong Li JCVI

By lazar
(234 views)

Next Generation Sequencing Data Analysis

Next Generation Sequencing Data Analysis

Next Generation Sequencing Data Analysis. Nadia Pisanti, University of Pisa. Why sequencing?. The knowledge of DNA and RNA sequences has become a crucial tool for: Basic research in biology, pharmacology and medicine.

By taffy
(521 views)

DNA Sequencing

DNA Sequencing

DNA Sequencing. Kabi R. Neupane, Ph.D. Leeward Community College ABE Workshop 2006. What to Sequence?. The RT-PCR product has been inserted into the pCR4-TOPO vector Our goal -Sequence the insert DNA. We Supplied. Template DNA: Your plasmid Primer DNA: T3 or T7. Frederick Sanger.

By chinue
(241 views)

Genomic DNA Cloning

Genomic DNA Cloning

Genomic DNA Cloning. Plasmid DNA Ligation. DNA Cloning. Shotgun sequencing di un intero genoma. Frammentazione DNA genomico in piccoli pezzi (ca.1000 bp) Clonaggio in plasmidi Sequenza dell’ inserto dalle due estremità Allineamento computerizzato delle sequenze Quanto sequenziare?

By flo
(825 views)

Genomics

Genomics

Genomics. DNA array s a nd chips (semi) qRT- PCR Northern blot + hybrid. Trans k rip tional fusions. 2D ele c tro phoresis Mass spectrometry Protein sequencing Translational fusional Immunodetection Enzyme activities. Regula tion. Chromatogra phy Mass spectrometry NMR.

By rupali
(154 views)

Biological Motivation for Fragment Assembly

Biological Motivation for Fragment Assembly

Biological Motivation for Fragment Assembly. Rhys Price Jones Anne R. Haake. What is fragment assembly?. The reconstruction of the contiguous chromosomal DNA sequence from short, experimentally-generated fragments.

By anoush
(157 views)

Why so hard for Classical Marine Biologists and Microbiologists?

Why so hard for Classical Marine Biologists and Microbiologists?

The Sorcerer II Global Ocean Sampling Expedition: Metagenomic Characterization of Viruses within Aquatic Microbial Samples

By wyman
(74 views)

Mohamed Tikah Marrakchi BIN6002 Summer, 2005

Mohamed Tikah Marrakchi BIN6002 Summer, 2005

Mohamed Tikah Marrakchi BIN6002 Summer, 2005. An Eulerian path approach to DNA fragment assembly Pavel A.Pevzner, Haixu Tang, and Michael S. Waterman PNAS 2001. Genome sequencing. Analysing DNA using improved technologies opens a new era in (but not only in) biomedical research.

By lorant
(139 views)

Chromosome 12

Chromosome 12

Chromosome 12.

By jenski
(76 views)

Perl Grabbag

Perl Grabbag

Some useful bits'n'pieces that every Perl programmer should know. Perl Grabbag. Strictness. #! /usr/bin/perl -w # bestrict - demonstrating the effect of strictness. use strict; $message = "This is the message.\n"; print $message;. Results from bstrict.

By eunice
(100 views)

THE GENOME

THE GENOME

THE GENOME. DESCRIBE THE MAIN FEATURES OF THE HUMAN GENOME INCLUDING DETAILS OF AT LEAST ONE SPECIFIC CHROMOSOME ELAINE WHITFIELD. THE HUMAN GENOME. The human genome actually consists of two genomes: - complex nuclear genome - simple mitochondrial genome. NUCLEAR

By dagan
(348 views)

GENOMA HUMANO, 26 Junho 2000

GENOMA HUMANO, 26 Junho 2000

GENOMA HUMANO, 26 Junho 2000. Celera. 175 mil leituras por dia 4 grupos 1- Transforma bactérias, praqueia e pega colonias 2- Mini-Prep 3- Reação de sequenciamento e precipitação com etanol 4- Alimenta os Sequenciadores ABI Prism 3700 65 pessoas. Celera.

By halil
(123 views)

Genomic Sequencing

Genomic Sequencing

Genomic Sequencing. DNA Sequencing. Fred Sanger, Cambridge, England Copy DNA with one of four bases starved ACGTAAGCTA with T starved produces ACG and ACGTAAGC Run experiment with each of four bases starved, producing a ladder (all sub-fragments ending at the base)

By navid
(137 views)

Sequencing a genome

Sequencing a genome

Sequencing a genome. Definition. Determining the identity and order of nucleotides in the genetic material – usually DNA, sometimes RNA, of an organism. Basic problem. Genomes are large (typically millions or billions of base pairs)

By brady-white
(90 views)

Lecture 6 Some useful bits & pieces that every Perl programmer should know

Lecture 6 Some useful bits & pieces that every Perl programmer should know

Lecture 6 Some useful bits & pieces that every Perl programmer should know. Strictness. Use strict;

By cathleen-guerrero
(88 views)

Venter et al.

Venter et al.

Community structure and metabolism through reconstruction of microbial genomes from the environment articles. Venter et al. Outline. Addressing : uncultivated microbial communities with unclear roles in natural system Source of Study: Acid Mine Drainage (AMD) microbial biofilm.

By adrian-woods
(192 views)

Today ’ s Lecture Topics Whole genome sequencing 						 Shotgun sequencing method

Today ’ s Lecture Topics Whole genome sequencing Shotgun sequencing method

Today ’ s Lecture Topics Whole genome sequencing Shotgun sequencing method Sequencing the human genome Functional/comparative genomics Transcriptome & RNA-Seq Proteomics. Shotgun DNA sequencing: Sequence the entire genome rapidly.

By glenna-murray
(188 views)

Today’s Topics

Today’s Topics

Today’s Topics. Computer Science Enabled by Computing : Decoding the Human Genome Upcoming Review for Final Exam. Enabled by Computers. Things we now take for granted: Possible only because of computing-- Several Examples (most mentioned before) Modern Camera Zoom Lens

By dale-dickson
(103 views)

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