'Small rnas' diaporamas de présentation

Welcome Each of You to My Molecular Biology Class

Welcome Each of You to My Molecular Biology Class. Molecular Biology of the Gene, 5/E --- Watson et al. (2004). Part I: Chemistry and Genetics Part II: Maintenance of the Genome Part III: Expression of the Genome Part IV: Regulation Part V: Methods. 2005-5-10. Part IV Regulation.

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Purifying DNA & RNA Source Amounts & Purity Damage or Loss

Purifying DNA & RNA Source Amounts & Purity Damage or Loss. Cells. Extract. Remove junk. Pure DNA. Bind DNA. 1. Remove high MW junk. DNA, RNA Solution. Denatured Protein. Phenol. Cell Extract. Shake. Spin. 2. Remove low MW junk and concentrate. Add. (to 70%). and salt.

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Mechanisms of Transcription

Mechanisms of Transcription. RNA polymerase does not need a primer to initiate transcription. The RNA product does not remain base-paired to the template DNA strand. Transcription is less accurate than replication. RNA polymerases and the transcription cycle.

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Reactions

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Noncoding Y RNAs: A Functional Role in DNA Replication

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RNA ???????

RNA ???????. ???. Transgenic gene silencing. ?????????? ???? gene knock out, knock down ???? gene therapeutics ??????. ???????? RNAi has be a powerful tool with which to dissect gene function in plant, C.elegans , Drosophila, and other lower eukaryote organisms

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Small RNAs and their regulatory roles. Presented by: Chirag Nepal

Small RNAs and their regulatory roles. Presented by: Chirag Nepal. Papers to be discussed. Tiny RNAs associated with transcription start sites in animals. Nat Genet. 2009 May;41(5):572-8. Epub 2009 Apr 19.

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Promoters and Enhancers

Promoters and Enhancers. Chapter 24. 24.1 Introduction. Figure 24.1. 24.2 Eukaryotic RNA Polymerases Consist of Many Subunits. RNA polymerase I synthesizes rRNA in the nucleolus. RNA polymerase II synthesizes mRNA in the nucleoplasm.

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MicroARNs, comparaison, prediction

MicroARNs, comparaison, prediction. Eric Bonnet Bioinformatics & Evolutionary Genomics Department of Plant Systems Biology Ghent University / VIB Belgium. Small RNAs biogenesis and function. Plan de la presentation. But de la presentation

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MicroARNs, comparaison, prediction

MicroARNs, comparaison, prediction. Eric Bonnet Bioinformatics & Evolutionary Genomics Department of Plant Systems Biology Ghent University / VIB Belgium. Small RNAs biogenesis and function. Plan de la presentation. But de la presentation

387 views • 27 slides

RNA Interference

RNA Interference. Hannon, Nature 418:244-251 Jacques et al, Nature 418:435-8 Carmichael Nature 418:379-380 Allshire, Science 297:1818-9. RNA Interference (RNAi). Double stranded RNA responsible for post-transcriptional gene silencing of the gene from which it was derived. SPECIFIC

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Advantages of C. elegans : 1. rapid life cycle 2. hermaphrodite 3. prolific reproduction

Advantages of C. elegans : 1. rapid life cycle 2. hermaphrodite 3. prolific reproduction 4. transparent 5. only ~1000 cells 6. laser ablation 7. complete cell lineage map 8. genetics. RNA interference: A type of gene regulation Involving small RNA molecules

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RNA interference (RNAi)

RNA interference (RNAi). white RNAi hairpin. Wild-type. A Brief History of RNAi. Hint 1995, Guo and Kemphues: Injection of par-1 gene sense RNA into the gonad of C. elegans induced par-1 null phenocopies at the same high frequency as injection of anti-sense RNA. First description

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Identifying and classifying functional small RNAs from pine

Identifying and classifying functional small RNAs from pine. Ryan Morin BC Genome Sciences Centre (presenting research conducted in the lab of Dr. Peter Unrau). Small RNAs in the land plants. MicroRNA Biogenesis. MicroRNA (miRNA) transcripts can be mono- or polycistronic

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Plant RNA silencing

the. Plant RNA silencing. Plant RNA silencing. Plant RNA silencing. Small RNAs mediate many regulatory processes in plants including: Post-transcriptional gene silencing, RNA-dependent DNA methylation , Protection from mobile genetic elements, Virus defence.

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Small RNA World microRNAs(miRNAs)

Small RNA World microRNAs(miRNAs). ECES458 FALL 2001 Student: Kai He Instructor: Dr. Sahinalp. imRNAs: A New Class of Small RNA Molecules. siRNAs: small interfering RNAs stRNAs: small temporal RNAs. siRNAs.

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SMALL RNAs IN RICE AND MAIZE

SMALL RNAs IN RICE AND MAIZE Lewis Bowman 1, Vicki Vance 1 , Rebecca Bullard-Dillard 2 , Cameron Johnson 3, * , Alex Adai 3, * , Venkatesan Sundaresan 3, *

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Clusters and Superclusters of Phased Small RNAs in the Developing Inflorescence of Rice

Clusters and Superclusters of Phased Small RNAs in the Developing Inflorescence of Rice Lewis Bowman 1, Vicki Vance 1 , Rebecca Bullard-Dillard 2 , Cameron Johnson 3, * , Alex Adai 3, * , Venkatesan Sundaresan 3, *

119 views • 1 slides