'Specific primers' diaporamas de présentation

Biotechnology

Biotechnology. Introduction. Biotechnology is essentially the use of living organisms and their products for health, social or economic purposes.

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GENETIC MARKERS IN PLANT BREEDING

GENETIC MARKERS IN PLANT BREEDING. Marker. Gene of known function and location, or a mutation within a gene that allows studying the inheritance of that gene Genetic information resides in the genome. Genetic Marker.

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Mid-term Examination

Mid-term Examination. On October 14 th , 2011 mid-term One hour in class exam, 100 points Closed book Essay type questions plus some short answers Will cover ALL chapters done until then Will count towards 30% of the final grade ( No chapter by chapter exams/quizzes until midterm ).

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Training on STR Typing Using Commercial Kits and ABI 310/3100

Training on STR Typing Using Commercial Kits and ABI 310/3100. Margaret C. Kline, Janette W. Redman, John M. Butler National Institute of Standards and Technology October 22-26, 2001. Human Identity Testing. Forensic cases -- matching suspect with evidence

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Exact and Approximation Algorithms for DNA Tag Set Design

Exact and Approximation Algorithms for DNA Tag Set Design. Ion Mandoiu and Dragos Trinca Computer Science & Engineering Department University of Connecticut. Overview. Background on universal tag arrays Tag set design problem c-h code formulation Integer program and LP-approximation

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DNA-barcoding on a different scale: challenges within Coccoidea

DNA-barcoding on a different scale: challenges within Coccoidea. Lyn Cook, The University of Queensland, Australia. SIBI Scale Insect Barcode Initiative (Stellenbosch, Feb 2007). Outline. What are scale insects? Why have they been a challenge? Prospects for a universal scale insect barcode.

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Treatment

Treatment. There is no specific treatment for smallpox and the only prevention is vaccination.

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The EBI search engine: EB-eye

The EBI search engine: EB-eye. Franck Valentin External Services group. EMBRACE Workshop CBS, BioCentrum-DTU, February 6-8, 2008. Summary. The data at the EBI What is the EB-eye? A glance at the web interface Web services for the EB-eye. ID ... AC ... DT . ID ... AC ... DT .

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Recombinant DNA Technology

Recombinant DNA Technology. Isolating DNA. C hemically, DNA is a very simple compound, with little variation between species. The basic steps: Break the cells open Disrupt cell membranes with a detergent Remove proteins and other macromolecules

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Regulation of MHC II Gene Transcription

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Monitoring of Minimal Residual Disease Principles and Applications

Monitoring of Minimal Residual Disease Principles and Applications. Pei Lin, MD Department of Hematopathology UT M.D. Anderson Cancer Center, Houston, TX. MRD studies in AML: Potential Utility. Definition: Residual disease morphologic complete remission (CR) ( ? 5% blasts)

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Novel Approaches & Probes for PCR Detection of Animal-Derived Materials

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Fig. S1

Fig. S1. Fig. S1 Representative histologic image of CCl 4 -induced cirrhotic liver. Azan-Mallory staining; original magnification x50. Fig. S2. Cirrhosis. Normal. IL-1 b. IL-8. TNF- a. TGF- b. PRL21. Fig. S2 Cytokine expression levels in the liver of ci rrhotic rats.

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Primer Design

Primer Design. Pool Design. Introduction. Sequence Data Complete Avian Influenza NA sequences from North America available in NCBI flu database [ Bao et al. 2008] as of March 2008 Phylogenetic analysis detected a N1 sequence mislabeled as N4

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Supplementary Table I . Gene-specific primers used for quantitatative real-time PCR

Supplementary Table I . Gene-specific primers used for quantitatative real-time PCR.

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a. b.

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qPCR measures quantities of target sequences

qPCR measures quantities of target sequences . Targets can be DNA, cDNA or RNA DNA : present in a sample ? number of copies in a sample ? -> copy number analysis ! Plasmids need to be linearized for qPCR cDNA : quantify expression levels -> gene expression analysis

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