Tryptic peptides - PowerPoint PPT Presentation

Proteomics and mass spectrometry

Proteomics and mass spectrometry. Manimalha Balasubramani. Outline. Mass spectrometers Protein identification Quantitative proteomics Protein-protein interactions. 1258.5603. 100. 1303.7007. 6.3E+4. 90. 80. 70. 60. 878.4913. % Intensity. 1031.5374. 983.4860. 50.

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Supplemental Fig. S1

Supplemental Fig. S1. a). b). PsAPY1 (AB071369) 1:MEFLIKLIT-FLLFSMPAITSSQYLGNNLLTSRKIFLKQEEISSYAVVFDAGSTGSRIHV 59 MtAPY1;1 (AY180377) 1:MEFLITLITTVLLLLMPAITSSQYLGNNLLTNRKIFQKQETISSYAVVFDAGSTGSRIHV 60 ***** *** ** **************** **** *** *******************

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32 P radioactivity ( cpm )

5000. 80. 50. 32 P radioactivity ( cpm ). Acetonotrile (% ). 2000. 30. 0. 0. 8000. 80. 5000. 50. 32 P radioactivity ( cpm ). Acetonotrile (% ). 30. 2500. 0. 14000. 80. 10000. 50. 32 P radioactivity ( cpm ). Acetonotrile (% ). 30. 5000. 0. 0. 10. 20. 30. 40.

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