'Yfp' diaporamas de présentation

The GFP revolution!

441 views • 43 slides

Let's return to the FRET experiment:

267 views • 20 slides

Transfection of alternative cell lines

Transfection of alternative cell lines. RAW 264.7 3T3-L1 J774 AtT-20. Expression of YFP in Electroporated WEHI 231 cells. Phase. CMV_YFP. 30-60% transfection efficiency. Expression of YFP in electroporated WEHI 231 and HEK293 cells. WEHI 231 293.

491 views • 18 slides

The principle of FRET

Potential combinations of CFP- and YFP-tagged CD147 and MCT1. The principle of FRET. N. -. terminus. OR. YFP. YFP. CFP. CFP. MCT1. s. s. CD147. s. s. Membrane. N. -. terminus. C. -. terminus. CFP. CFP. YFP. YFP. YFP. YFP. CFP. CFP. OR. OR. CFP. CFP. YFP. YFP.

323 views • 7 slides

Effector

0. 100. 200. 300. 404. 500. Effector. CaMV 35S. RAP2.4a. 511%. Empty vector. CaMV 35S. single cell fluorometry. -1425. +9. Reporter. YFP. 2CPA-wt-Promoter. Relative Activity (% YFP/CFP). A. Reference. CFP. CaMV 35S. TT. -1425. +9. mut-Promoter. YFP. 2CPA-mut-Promoter.

147 views • 1 slides

YFP

Light. YFP. Auto. Merge. MsTPS1-YFP. MsTPS2-YFP. A. CFP. YFP. Merged. Light. MsTPS1-YFP + CFP. MsTPS2-YFP + CFP. B. Additional file 9.

128 views • 1 slides