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CSC Spheroid Isolation

Supplemental Figure 1. Addition of controls and MIPE to both. A. CSC Spheroid Isolation. Dissociation of spheroids/ Clonal seeding into assay plate. 7-14 days. Spheres. 2 days. Measure Cell viability (CellTiterGlo TM ). SCM+KO+ITS. SCM+KO+ITS. B. Cell Seeding. DMEM or RPMI+5%FBS.

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CSC Spheroid Isolation

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  1. Supplemental Figure 1 Addition of controls and MIPE to both A CSC Spheroid Isolation Dissociation of spheroids/ Clonal seeding into assay plate 7-14 days Spheres 2 days Measure Cell viability (CellTiterGloTM) SCM+KO+ITS SCM+KO+ITS B Cell Seeding DMEM or RPMI+5%FBS Adherent C D

  2. Supplemental Figure 2 ≤30% viability in both LNCaP/PANC1 ≤30% viability in Sphere ≤30% viability in both Sphere/Adherent ≤30% viability in Adherent ≤ 30% viability in PANC1 viability ≤30% in LNCaP 2 19 5 7 2 28

  3. Supplemental Figure 3 Active CRC in both LNCaP/PANC1 Active CRC CRC in Adherent Active CRC CRC in Sphere Active CRC in both Sphere/Adherent Active CRC in PANC Active CRC in LNCaP 5 5 31 16 8 24

  4. A B IC50 (mM) 12.5 IC50 (mM) 1.481 C Cell Line CRC IC50 (mM) LNCaP A -1.2 0.007 LNCaP S -1.1 0.04 PANC1 A -1.4 0.03 PANC1 S -1.4 0.01 Supplemental Figure 4

  5. Cell Line CRC IC50 (mM) LNCaP A -2.4 45.6 LNCaP S -2.1 40.7 PANC1 A -2.4 1 PANC1 S -1.4 45.6 Supplemental Figure 5

  6. Supplemental Table 1

  7. Supplemental Table 2

  8. Supplemental Table 3

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