Institute of Living Systems

1. Institute of Living Systems

2. Institute of Living Systems • Neuroscience Center • Laboratory of Stem Cells Research and Regenerative Medicine • Laboratory of Experimental Oncology • Laboratory of Radiation Biology • Laboratory of Electrophysiology and Modeling of the Heart • Laboratory of Plant Electrophysiology • Laboratory of Biological and Medical Chemistry • Center of Radiophysical Methods in Biomedicine • Center of Mathematical Simulation • SPF-vivarium

3. Institute of Living SystemsNeuroscience Center Mission: Science. Understanding mechanisms and principles of brain functions: from molecular to cognitive levels. The development of neuromorphic intelligence Technologies. Optical and electrophysiological monitoring, computer simulations and virtual neural networks, neurointerfaces and neurally controlled robots Laboratories: • Molecular Neurobiology • Cellular neurotechnologies • Extrasynaptic regulation • Extracellular matrix of the brain • Neuroanimats: technologies of neurohybrid systems • Mathematical modeling: virtual neural networks • Cellular electrophysiology • Cognitive neurotechnologies • Neuron-glial networks

4. Institute of Living SystemsNeuroscience Center • Extracellular matrix – extracellular molecular network accompanying synapses and modulating synaptic transmission and plasticity Megagrant «Extracellular matrix as a determinant of intercellular communication and a target for therapeutic interventions» SchizophreniaNprmal Leading scientist: Prof. Alexander Dityatev (Italian Institute of Technologies, Genova, Italy) • Molecular neurobiology Perineuronal net in normal conditions and for schizophrenia • Cellular electrophysiology

5. Institute of Living Systems Neuroscience Center • “Brain in a chip” – living cells on multielectrode arrays. The development of neuroanimats Rat or mouse brain (cortex or hippocampus) Dissociated cells • Growing virtual neurons and neuronal nets in computer simulation Multielectrode probe

6. Institute of Living SystemsLaboratory ofStem cells research and regenerative medicine Tumor model • Tumor – mesenchimal stem cells interaction investigation by fluorescence bioimaging • Investigation of functional activity of Induced pluripotent stem cells in whole body RFP labelled mesenchimal Stem cells Stem cells inside the tumor

7. Institute of Living SystemsLaboratory ofStem cells research and regenerative medicine • Development of cells culture system for Bioreactor “Artificial liver”, development Scafolds. • Cell surgery with femtosecond laser system Lung metastasis with GFP stem cells RFP stem cells inside the tumor Confocal microscopy (Carl Zeiss, Germany)

8. Institute of Living SystemsLaboratory of Experimental oncology • Fundamental studies of carcinogenesis and molecular mechanisms of cancer response to treatment. • Development of biocompartible contrasting agents for optical methods of cancer diagnostics. Photosensitizers uptake by cancer cells Fluorescence whole-body imaging of small animal tumor models Visualization of heart muscle vasculature using quantum dots Cancer cells expressing red fluorescent protein

9. scFv-antibodies Barstar Barnase Quantum dot Institute of Living SystemsLaboratory ofExperimental oncology • Molecular diagnostics of cancer based on targeted delivery of contrasts . Individualization of treatment Quantum dots-based complexes for targeted labeling of cancer cells • Novel optical methods for screening of antineoplastic drugs • Development of new generation drugs for chemotherapy and photodynamic therapy of cancer Day 6 Day 10 Day 17 Intravital monitoring of tumor growth by fluorescence imaging method

10. Institute of Living SystemsLaboratory ofRadiation biology Oxygenated tumor Hypoxic tumor • Study of tumor oxygen state and oxygen-modifying agents for radiation therapy Diffuse optical spectroscopy • Study of mechanisms of radiation-induced damage of normal tissues, methods of prophylaxis and treatment • Study of biological effectiveness of hadron therapy (proton and boron-capture therapy) using particle beams from alternative sources)

11. Institute of Living SystemsLaboratory ofElectrophysiology and Modeling of the Heart Arrhythmology • Investigation of mechanisms of cardiac arrhythmias • Creation of new methods of defibrillation Computational Cardiology • Creation of software of cardiologist "Virtual Heart“: • 3D high-realistic model of main parts of human heart and software for modeling heart activity (both healthy and diseases cases)

12. Institute of Living SystemsLaboratory ofPlant Electrophysiology • Investigation of mechanisms electric signals in plants • Functional role of electrical signals in plants • Mathematical simulation of electrogenesis • Study of the effects of physical factors - ionizing radiation and electromagnetic radiation - on higher plants • Development of diagnostic methods of the functional state of higher plants

13. Institute of Living SystemsLaboratory ofBiological and Medical Chemistry • Synthesis of tubulin-binding anticancer molecules acting on colchicine-site • Elaboration of liposomal drug-delivery systems • Construction of fluorescent markers

14. Institute of Living SystemsCenter of Radiophysical Methods in Biomedicine Mission: Development of methods and equipment for diagnostics of biological objects structure and functions, and precision control of biosystems behavior Laboratories: «Acoustic methods of diagnostics and treatment monitoring» «Microwaves spectroscopy and radiometry» «Laser methods of living systems research» «Biophotonics»

15. Institute of Living Systems Center of Mathematical Simulation Laboratories: • «Mathematical problems of biology» • «Complex biostructures» • «Biomedical imaging» • Nonlinear dynamics of living systems and bioinformatics • Research of fractal structures and structural phase transitions in biological and ecological systems • 3D stereo-visualization, 3D reconstruction, geometric simulation in biomedicine