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THE HYPERSPECTRAL IMAGING APPROACH Giuseppe Bonifazi Workshop

THE HYPERSPECTRAL IMAGING APPROACH Giuseppe Bonifazi Workshop. ACTIONS C.1&C.2 - Process and product monitoring based on hyperspectral imaging.

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THE HYPERSPECTRAL IMAGING APPROACH Giuseppe Bonifazi Workshop

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  1. THE HYPERSPECTRAL IMAGING APPROACH • Giuseppe Bonifazi • Workshop

  2. ACTIONS C.1&C.2 -Process and product monitoringbased on hyperspectral imaging HyperSpectral Imaging (HSI) is an innovative technology developed in recent years that combines the advantages of spectroscopy and imaging techniques. In the last years there was a strong increase in applications including off-line / on-line inspection at industrial scale in different sectors. Image source: www.perception-park.com RESAFE Project Workshop Faenza (Italy) December 14, 2015

  3. ACTIONS C.1&C.2 -Process and product monitoringbased on hyperspectral imaging Spectral signature of a single pixel All individual spatial and spectral images could be picked up from the hypercube and the spectrum of each pixel of the image in a specific position can be extracted One spectral image of n pixels for all the investigated wavelengths (λ) RESAFE Project Workshop Faenza (Italy) December 14, 2015

  4. ACTIONS C.1&C.2 –The selected HSI devices Hyperspectral Imaging Characterization SISUChema XL™ Chemical Imaging Workstation • Operation mode: push-broom • Spectral range: SWIR: 1000-2500nm • Spectral sampling and (resolution) 6.3nm • Active pixel: 320 (spatial) x 240 (spectral) pixels RESAFE Project Workshop Faenza (Italy) December 14, 2015

  5. ACTIONS C.1&C.2 –The selected HSI devices Hyperspectral Imaging Characterization Hyperspectral architecture Specim ImSpectorTM N17E Hyperspectral architecture Specim ImSpectorTM V10 • Operation mode: push-broom • Spectral range: NIR: 1000-1700nm • Spectral sampling and (resolution) 5nm • Active pixel: 320 (spatial) x 240 (spectral) pixels • Operation mode: push-broom • Spectral range: VIS-NIR: 400-1000 nm • Spectral sampling and (resolution) 5 nm • Active pixel: 320 (spatial) x 240 (spectral) pixels RESAFE Project Workshop Faenza (Italy) December 14, 2015

  6. Actions C.1-C.2 - Sample Acquisition ACTION C.1: HyperSpectral Imaging (HSI) Characterization RESAFE Project Workshop Faenza (Italy) December 14, 2015

  7. Actions C.1-C.2: Hyperspectral data processing Spectral data have been analysed using the PLS_Toolbox 7. 0 (Eigenvector Research Inc.) running inside Matlab™ environment (version 7.12.0). • Spectra preprocessing • PCA applied for data exploration • PLS applied for spectra correlation with curing time and different measured chemical parameters RESAFE Project Workshop Faenza (Italy) December 14, 2015

  8. Actions C.1&C.2 – Results on raw materials from the different countries (Action B.1) IT-FOR Sample Step 1: Waste raw materials collected from Italy, Spain and Cyprus were utilized to train the HSI system to correctly classify them. Step 2: Hyperspectral images of different organic waste were elaborated using the 3 different HSI devices. Step 3: Spectra preprocessing and PCA classification model were applied for the classification of different organic matrices. CY-UOW Sample RESAFE Project Workshop Faenza (Italy) December 14, 2015 1 cm a) b) c) a) Raw Hyperspectal images of a) IT-BC; b) IT-FOR and c) IT-UOW b) c) Applied pretreatment: • SNV • Mean Center

  9. Actions C.1&C.2 – Results on raw materials from the different countries (Action B.1) Raw materials: Italian samples Reflectance spectra (1000-1700 nm) RESAFE Project Workshop Faenza (Italy) December 14, 2015

  10. Actions C.1&C.2 – Results on raw materials from the different countries (Action B.1) Italian Raw Materials a) b) c) a) b) c) Raw Hyperspectal images of a) IT-BC; b) IT-FOR and c) IT-UOW Applied pretreatment: • SNV • Mean Center a) b) c) a) IT-BC c) IT-UOW Pretreated Hyperspectal images of a) IT-BC; b) IT-FOR and c) IT-UOW b) IT-FOR RESAFE Project Workshop Faenza (Italy) December 14, 2015

  11. Actions C.1&C.2 – Results on raw materials from the different countries (Action B.1) PCA score plots (PC1 vs. PC2) related to the different FOR samples coming from Italy, Spain and Cyprus acquired in the NIR (1000-1650 nm) wavelength field. RESAFE Project Workshop Faenza (Italy) December 14, 2015

  12. Actions C.1&C.2 – Results from lab-scale plantsin the different countries (Action B.2) Italian samples at different curing times Average reflectance spectra of the different Italian samples acquired in the NIR (1000-1650 nm) wavelength field RESAFE Project Workshop Faenza (Italy) December 14, 2015

  13. Actions C.1&C.2 – Results from lab-scale plantsin the different countries (Action B.2) Italian samples at different curing times PCA score plots (PC1 vs. PC2) related to the different Italian samples (IT-UFVB-5050-T0; IT-UFVB-5050-T20; IT-UFVB-5050-T40 and IT-UFVB-5050-T60) acquired in the NIR (1000-1650 nm) wavelength field RESAFE Project Workshop Faenza (Italy) December 14, 2015

  14. Actions C.1&C.2 – Results from lab-scale plantsin the different countries (Action B.2) Process Monitoring by HSI PLS for different curing times (Italian samples) t = 60 days t = 40 days t = 20 days t = 0 days RESAFE Project Workshop Faenza (Italy) December 14, 2015

  15. Actions C.1&C.2 – Results from lab-scale plantsin the different countries (Action B.2) Product Monitoring by HSI Prediction of chemical parameters (Italian samples) Humidity (%) Phosphatase (µmol INTF g-1 h-1) RESAFE Project Workshop Faenza (Italy) December 14, 2015

  16. Actions C.1&C.2 – Results from lab-scale plantsin the different countries (Action B.2) Product Monitoring by HSI Prediction of chemical parameters (Italian samples) Soluble Organic Carbon (%) Soluble Organic Nitrogen (ppm) RESAFE Project Workshop Faenza (Italy) December 14, 2015

  17. Actions C.1&C.2 – Results from pilot-scale plantsin the different countries (Action B.3) Product Monitoring by HSI PCA (Spanish samples) Source Sample ES-UFVB-6040 T90 Sample Hypercube PCA: background removal RESAFE Project Workshop Faenza (Italy) December 14, 2015

  18. Actions C.1&C.2 – Results from pilot-scale plantsin the different countries (Action B.3) Process Monitoring by HSI Raw and preprocessed spectra at t = 0 and t = 90 days (Spanish samples) Average raw spectra Preprocessing applied: Smoothing (window 11)+ Second derivative (window 15)+ Mean center RESAFE Project Workshop Faenza (Italy) December 14, 2015

  19. Actions C.1&C.2 – Results from pilot-scale plantsin the different countries (Action B.3) Process Monitoring by HSI PCA and Loadings (Spanish samples) RESAFE Project Workshop Faenza (Italy) December 14, 2015

  20. Actions C.1&C.2 – Results from pilot-scale plantsin the different countries (Action B.3) Product Monitoring by HSI Prediction of chemical parameters: Soluble Organic Nitrogen (%) Spanish samples Italian samples RESAFE Project Workshop Faenza (Italy) December 14, 2015

  21. Actions C.1&C.2 – Results from pilot-scale plantsin the different countries (Action B.3) Product Monitoring by HSI Prediction of chemical parameters: Soluble Organic Carbon (%) Spanish samples Italian samples RESAFE Project Workshop Faenza (Italy) December 14, 2015

  22. Actions C.1&C.2 – Results from pilot-scale plantsin the different countries (Action B.3) Product Monitoring by HSI Prediction of chemical parameters: EC (mS/cm) Spanish samples Italian samples RESAFE Project Workshop Faenza (Italy) December 14, 2015

  23. Actions C.3 – Results from agriculture application in Italy (Action B.4) Product Monitoring by HSI PCA (Italian samples) - Barley RESAFE Project Workshop Faenza (Italy) December 14, 2015

  24. Actions C.3 – Results from agriculture application in Italy (Action B.4) Process Monitoring by HSI Raw and preprocessed spectra Preprocessing applied: Smoothing (window 15)+ First derivative (window 15)+ Mean center Average raw spectra RESAFE Project Workshop Faenza (Italy) December 14, 2015

  25. Actions C.3 – Results from agriculture application in Italy (Action B.4) PCA score plots (PC1 vs. PC2) related to Barley samples acquired in the NIR (1000-1650 nm) wavelength field. RESAFE Project Workshop Faenza (Italy) December 14, 2015

  26. Actions C.4 – Results from agriculture application in Spain (Action B.5) Product Monitoring by HSI PCA (Spanish samples) - Potato RESAFE Project Workshop Faenza (Italy) December 14, 2015

  27. Actions C.4 – Results from agriculture application in Spain (Action B.5) Process Monitoring by HSI Raw and preprocessed spectra Preprocessing applied: Smoothing (window 15)+ Second derivative (window 15)+ Mean center Average raw spectra RESAFE Project Workshop Faenza (Italy) December 14, 2015

  28. Actions C.4 – Results from agriculture application in Spain (Action B.5) PCA score plots (PC1 vs. PC2) related to Potato samples acquired in the NIR (1000-1650 nm) wavelength field. RESAFE Project Workshop Faenza (Italy) December 14, 2015

  29. Actions C.5 – Results from agriculture application in Cyprus (Action B.6) Product Monitoring by HSI PCA (Cypriot samples) - Melon RESAFE Project Workshop Faenza (Italy) December 14, 2015

  30. Actions C.5 – Results from agriculture application in Cyprus (Action B.6) Process Monitoring by HSI Raw and preprocessed spectra Preprocessing applied: Smoothing (window 11)+ Second derivative (window 19)+ Mean center Average raw spectra RESAFE Project Workshop Faenza (Italy) December 14, 2015

  31. Actions C.5 – Results from agriculture application in Cyprus (Action B.6) PCA score plots (PC1 vs. PC2) related to Potato samples acquired in the NIR (1000-1650 nm) wavelength field. RESAFE Project Workshop Faenza (Italy) December 14, 2015

  32. Conclusions Actions B.1&C.1-C.2 The HSI approach allowed to outline the difference existing among the investigated raw material samples both in terms of composition (i.e. UOW, FOR and BC) and origin (i.e.: Italy, Spain and Cyprus), with the only exception of Italian and Spanish BC. Actions B.2&C.1-C.2 Goodcorrelationbetweenspectralbehavior in the NIR wavelengthrange of the differentcollectedsamples from the lab-scale plantsin the differentcountries and curing time and the mainmeasuredchemicalparameters (humidity, soluble carbon, solublenitrogen, and phosphatase) wereobtained. Actions B.3&C.1-C.2 Goodcorrelationbetweenspectralbehavior in the NIR wavelengthrange of the differentcollectedsamples from the pilot-scale plantsin the differentcountriesand the mainmeasuredchemicalparameters(solublecarbon, solublenitrogen and electricalconductivity) wereobtained. RESAFE Project Workshop Faenza (Italy) December 14, 2015

  33. Conclusions Actions B.4&C.3 The HSI approach allowed to outline the difference crops existing from different thesis in the agriculture application in Italy. Actions B.5&C.4 The HSI approach allowed to outline the difference crops existing from different thesis in the agriculture application in Spain. Actions B.6&C.5 The HSI approach allowed to outline the difference crops existing from different thesis in the agriculture application in Cyprus. RESAFE Project Workshop Faenza (Italy) December 14, 2015

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