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This study investigates the role of SIRT-1 and PGC-1α siRNA in modulating cellular responses to leucine. Using various controls and negative controls, we analyze the impact of these siRNAs on gene expression levels across SIRT-1, PGC-1α, and NRF, measured against 18S rRNA. The findings aim to elucidate the molecular mechanisms underpinning leucine's effects on metabolic regulation and identify potential therapeutic targets for metabolic disorders.
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A * * * SIRT-1/18S siRNA negative control Control siRNA siRNA leucine B C * * * * * * PGC-1α/18S NRF/18S Control siRNA negative control siRNA siRNA leucine Control siRNA negative control siRNA leucine siRNA