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Design of a Mycobacterial Porin Based Dye Sensitized Solar Cell

Design of a Mycobacterial Porin Based Dye Sensitized Solar Cell. Ayomi S. Perera*, Mausam Kalita*, Navaneetha K. Subbaiyan † , Francis D ’ Souza † and Stefan H. Bossmann* Kansas State University*, Wichita State University †. Objective :. Experimental Methods. Background.

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Design of a Mycobacterial Porin Based Dye Sensitized Solar Cell

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  1. Design of a Mycobacterial Porin Based Dye Sensitized Solar Cell Ayomi S. Perera*, Mausam Kalita*, Navaneetha K. Subbaiyan†, Francis D’Souza† and Stefan H. Bossmann* Kansas State University*, Wichita State University† Objective : Experimental Methods Background To produce a ‘green’ dye sensitized solar cell with a protein electrolytic matrix • MspA is a trans-membrane • channel-forming protein from • Mycobacterium smegmatis • It mediates transport of ions • across the cell membrane • Octamer, 160 kDa • Extremely stable: 100oC in SDS & pH 2-10 • Contains 96 free –COOH & • 8 -NH2’s • MspA mutant A96C contains • cysteine at terminal of • docking zone • MspA liquid extracts & crystals can • be obtained in high yield • It can be characterized by methods • such as gel electrophoresis & HPLC Ru (1) Ru (2) M. smegmatis http://en.wikipedia.org/wik i/File:Msmegmatiswiki.jpg MspA Niederweis, M. Molecular microbiology 2003, 49(5), 1167-1177 Ru (1) or Ru (2) EDC coupling • Adsorb onto TiO2 • Expose to sunlight • Measure voltage & current A96C MspA with terminal cysteine + Ru based dye Maleimide linker Electron Excitation TiO2 Nano Particles Cysteine Faller, M. et al. Science 303, 1189 (2004) Double bond of maleimide reacts with –SH of cysteine MspA Gel electrophoresis Experimental Methods Results & Conclusion HPLC MspA liquid extract MspA crystal 1) 0.385 mmol 0.07 g, 1.2 eq MspA is Highly Versatile! EDC 1.23 mmol, 1.5 eq HOBT 0.82 mmol, 1 eq 0.82 mmol 0.15 g, 1 eq. (X) • Cytotoxic • Drug Delivery System for Cancer Treatment • Stable Supramolecular Building Unit • Improved Cure for Tuberculosis • Nano reactor for Synthesis of Gold-Nanoparticles, Nano-dots and Nano-antennae • Defined Protein-Matrix for Vectorial Electron-Transport in Solar Cells • DNA Sequencing Using Channel Activity • Quenching Heavy Metals from Biological Systems • Cool to r.t. • Add 25 ml H2O • Heat 100 oC, 2h Reflux 30 min 2) RuCl3.3H2O + DMSO 0.38 mmol 4 ml 0.1 g Ru(DMSO)4Cl2 (pale yellow) • Cool to 60 oC [Ru(DMSO)4-n(H2O)n]2+ 2. Add X dropwise over 1h, at 40 oC, in EtOH/H2O 1% Conversion of sunlight into current First ever ‘Protein Nano Solar Cell’! • Stir intensively at 60 oC 10h • Add 0.55 mmol (0.100 g) of 1,10-phenanthroline-5-amine, keep at 60 oC, 1h • 3. Cool to r.t. • 4. Evaporate excess solvent • 5. Check TLC : two compounds • 6. Separate by Al2O3 column • 7. Characterize by 1HNMR & mass spectra 3) Ru (1) & Ru (2) Acknowledgement: National Science Foundation under Award No. EPS-0903806 and matching support from the State of Kansas through Kansas Technology Enterprise Corporation Pre-program Medication Errors (Jan-Sep 2006; n=191)

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