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Alternative Application Technology

Alternative Application Technology. Daniel L. Kline USDA-ARS, CMAVE Gainesville, FL dkline@gainesville.usda.ufl.edu. Alternative ApplicationTechnology. Area repellents Tent studies Insecticide-treated vegetation Semi-field: CMAVE protocol Field: Studies in Arkansas versus Anopheles.

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Alternative Application Technology

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  1. Alternative Application Technology Daniel L. Kline USDA-ARS, CMAVE Gainesville, FL dkline@gainesville.usda.ufl.edu

  2. Alternative ApplicationTechnology • Area repellents • Tent studies • Insecticide-treated vegetation • Semi-field: CMAVE protocol • Field: Studies in Arkansas versus Anopheles

  3. MilitaryRelevance Pristine Sand Fly Habitat … Everywhere Before Tent Cities

  4. Army Tent City Air Force Tent City

  5. Area Repellents: Tent Studies Metofluthrin (SumiOne™) A highly volatile pyrethroid

  6. Participants • CMAVE • Dan Kline, Jerry Hogsette and Brian Quinn • Joyce Urban and Melissa Doyle • NECE • CDR Steve Rankin • LCDR Dan Szumlas • Dr. Todd Walker • Valent • John Lucas

  7. Tent Studies: Metofluthrin (Sumi OneTM) • Types of emanators • Placement of emanators • Number of emanators • Environmental effects

  8. TENT A TENT B 100 feet 30 ft wide 60 ft long 13’ 6” wide 18’ 6” long West Cage East Cage Tent configurations

  9. SumiOne™ Paper Emanators Surface area 4000 cm3 (both faces) Treated with 200 mg SumiOne®

  10. Placement of emanators

  11. SumiOne™ Plastic Emanators 7.9 cm wide x 11 cm long (192 holes, each .25 cm2) Plastic impregnated with 5% (w/w) SumiOne®

  12. Mosquito Magnets and emanators

  13. Mosquitoes Releasedin Each Cage Ochlerotatustaeniorhynchus (500) Aedesaegypti (500)

  14. Measures of Emanator Efficacy • Landing counts on human volunteers (inside and outside tent) • Mosquito Magnet Pro collections (within tents) • Residue analysis

  15. Percent reduction in trap collections with SumiOne paper at two heights

  16. Percent residue of metofluthrin in paper emanators

  17. Average percent reduction in trap collection using SumiOne plastic emanator

  18. Outside Outside Inside Inside Untreated Treated

  19. Treated Untreated Nets

  20. Overall Summary • SumiOne® shows potentially good knockdown and bite inhibition activity against mosquitoes. • 2. Type and placement of SumiOne emanators is very important. • 3. Further research is needed on type and placement of emanators. • 4. Additional candidate compounds need to be evaluated.

  21. Large outdoor screen cage (16’ high) West 60’ Coleman MD-2500 10’ X X X X X X X 10’ North South 30’ X X X X X X X X X X X X X X X X X X X X X East Wax myrtle (Myrica cerifera) 7 plants per side

  22. Participants • CMAVE • Dan Kline, Jerry Hogsette, Sandy Allan and Brian Quinn • Joyce Urban, Aaron Lloyd and Melissa Doyle • Arkansas • David Dame (Entomological Services) • Max Meisch (University of Arkansas)

  23. CMAVE Protocol Mini-plots

  24. Bifenthrin residual treatment on wax myrtle

  25. Arkansas Studies David Dame (Entomological Services) and Max Meisch, Univ. of Arkansas Mini-plots (10’ x 10’) 0.1 acre plots Bifenthrin .22 lb/acre Bifenthrin .11 and .22 lb/acre

  26. Trap Collections

  27. Bifenthrin treated mini-plots

  28. Bifenthrin treated field plots

  29. Bioassay

  30. Percent mortality of An. quadrimaculatus after 1 hr exposure to Bifenthrin-treated leaves from field plots

  31. Percent mortality of An. quadrimaculatus after 24 hour exposure to Bifenthrin (Talstar) treated leaves from field plots.

  32. Percent mortality of Oc. taeniorhyncus after one hour exposure to Bifenthrin (Talstar) treated leaves from field plots.

  33. Percent mortality of Oc. taeniorhyncus after 24 hour exposure to Bifenthrin (Talstar) treated leaves from field plots.

  34. Residue Analysis

  35. Future Studies Comparison of regular backpack sprayers with electrostatic sprayers

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