THE UNIVERSITY OF TEXAS AT TYLER

1. THE UNIVERSITY OF TEXAS AT TYLER Institutional Animal Care and Use Committee Education: The Animal Care Technician *Note: Content not specific to UT Tyler is from the American Association for Laboratory Animal Science (AALAS)

2. The Animal Research Environment • All animals used for research and non-research purposes at UT Tyler are handled and cared for according to federal regulations. • The document that establishes these regulations is called The Guide for the Use and Care of Animals. • Other regulations that UT Tyler must follow are established by the United States Department of Agriculture (USDA) and the US Government Principles • The Office of Laboratory Animal Welfare (OLAW) is the federal agency in charge of enforcing federal regulations

3. Guide for the Care and Use of Laboratory Animals • The Guide for the Care and Use of Laboratory Animals is a booklet prepared by The Institute of Laboratory Animal Resources (ILAR), and is based on the US Public Health Service regulations for animal care and use. • The purpose of the Guide, first published in 1963 and last revised in 1996, is to help institutions address issues that concern the humane care, use and maintenance of laboratory animals. • The Guide outlines and references adequate veterinary care, facility environment and housing requirements, personnel qualifications, sanitation standards, surgical and postsurgical care, acceptable euthanasia techniques and facility construction guidelines.

4. The Animal Welfare Act • The Guide and UT Tyler regulations are based on The Animal Welfare Act: • Groups or institutions that sell, auction, exhibit, transport, breed or conduct research with animals are subject to regulation by the Animal Welfare Act. • The Animal Welfare Act regulates the use of all warm-blooded vertebrates in research except birds, rats and mice bred exclusively for research and farm animals used or intended for use as food or fiber. The Act also excludes farm animals intended for use in studies to improve animal nutrition, breeding, management or production efficiency. The Animal Welfare Act covers all wild mammals, even wild rats and mice. • The regulations deal with housing, handling, feeding, watering, sanitation, ventilation, transportation, separation of species and veterinary care for these animals.

5. The Research Facility Environment • The goal of research is to gain new knowledge with which to improve the future of people and animals. This research requires personnel, money to pay for supplies, salaries, animal care, equipment and facilities. • The largest source of government funding for biomedical research comes from the National Institutes of Health (NIH) as grants-in-aid. • Other government agencies, such as the National Science Foundation, the Environmental Protection Agency and some state and local government agencies also provide grants for biomedical research.

6. The Research Grant • Grants from local, state, and federal organizations provide money to purchase the equipment and labor necessary to carry out the study. A portion of this money covers the costs of animal care at a laboratory animal facility. • Granting agencies, institutions and scientists have a vested interest in making sure that the services and animal care provided by the laboratory animal facility and its staff are appropriate and cost effective.

7. The Research Proposal Before a research program can begin, the scientist must write a detailed outline of the proposed research. This document, referred to as a research proposal, explains: • Specific aims of the research study • Members of the research team • Expected results of the research • How the research will be beneficial • Describes what methods/procedures will be used to accomplish these aims (the protocol)

8. The Research Proposal and the IACUC • The animal protocol is a description of specific procedures to be done during the research study, and is a very important part of the proposal. • The protocol is the focus of review by a committee at UT Tyler called the Institutional Animal Care and Use Committee (IACUC). • This group of persons determines if the use of animals is appropriate, and ensures that the rights of animals are protected throughout the study.

9. The IACUC • The IACUC reviews all protocols that require the use of animals, and approval of the protocol by the IACUC is required before any animal work can begin. • The IACUC must base its decisions on fundamental scientific principles as well as the laws and regulations that govern the use of animals research. • If a proposed protocol fails to meet these criteria, the IACUC can either require the investigator to make modifications or can refuse to permit the project to begin or continue.

10. The IACUC • The UT Tyler IACUC reports to the Institutional Official (IO) who is appointed by the President of the University • The IO for UT Tyler is Dr. Michael Odell, Director of Research.

11. The Institutional Animal Care and Use Committee The IACUC is responsible for: • Overseeing animal research programs at UT Tyler • Reviewing research protocols that require the use of animals to be sure the methods of animal care and use are appropriate and in compliance with federal regulations and UT Tyler policies • Inspecting animal facilities and evaluating animal care programs twice a year • Reporting their findings and plans for correction of deficiencies to the Institutional Official • Serving as an information resource on animal welfare concerns for institutional personnel and the community at large

12. IACUC Legal and Moral Responsibilities • The Committee includes a veterinarian with experience in laboratory animal medicine, scientists from within the institution who use research animals, a nonscientist and a person not affiliated with the institution. • In the United States, the legal and primary moral responsibilities for good animal care rest with the institution itself. • At UT Tyler, the IACUC and Institutional Official can exert the necessary controls to ensure quality animal care. An IACUC that fails to comply with its duties can cost the institution its ability to receive public funding for animal research.

13. UT Tyler’s Animal Occupational Health and Safety Program Federal regulations and UT Tyler policies mandate that any employee working in laboratory animal facilities or with substantial animal contact be enrolled in an Animal Occupational Health and Safety Program

14. UT Tyler’s Animal Occupational Health and Safety Program • This includes persons involved in direct care of animals and/or their living environments • This also includes persons with periodic contact with animals (live or sacrificed), their viable tissues, body fluids, or wastes

15. UT Tyler’s Animal Occupational Health and Safety Program This policy then will include researchers, educators, vivarium staff, laboratory assistants, physical plant and housekeeping employees, and at times, students, visitors and guests.

16. UT Tyler’s Animal Occupational Health and Safety Program • Initiation into the occupational health program involves completing the Health Risk Survey, submitting it to the Director of UT Tyler’s Environmental Health and Safety department • After review by a health care professional, the employee may be cleared for animal contact, or may be called in for recommendations to see a their personal health care provider for immunizations or other intervention to minimize adverse health problems

17. UT Tyler’s Animal Occupational Health and Safety Program • Personnel must do an update survey every three years, or more frequently depending on the risk level. • All personnel are to be familiar with details concerning this program in the UT Tyler IACUC Policy Handbook

18. The Research Team • The size of research teams vary, depending on the type of investigation and the resources available. Typically, the team includes the principal investigator, co-investigators, research technicians, the laboratory animal veterinarian, and laboratory animal technicians. • The principal investigator, or PI, is the scientist who plans and coordinates all phases of the research work. He or she develops an idea and prepares the protocol. The PI, co-investigators, and research technicians perform the experiment and interpret the data. The PI is responsible for reporting the research findings to the scientific community.

19. Members of the Research Team • Co-investigators and research technicians usually perform the specific research tasks required for the protocol. These include observing the experimental animals, performing laboratory tests, and assisting with preparations. These team members also collect, organize and analyze the data generated from the research protocol. • Depending on their level of training and knowledge, animal technicians may perform some of these functions.

20. Laboratory Animal Technicians Animal technicians are essential members the research team. They perform many animal care functions critical to the maintenance of healthy animals. By doing this they also can control undesirable factors that adversely affect the research project. Such factors include: • Cleaning schedules • Sanitation procedures • Feeding • Bedding • Humidity • Heat • Light • Noise

21. The Animal Care Technician If improperly controlled, variations in any of these factors can contribute to physiological changes, disease debilitating conditions in laboratory animals. Animal technicians should make sure animals get proper medical care by alerting veterinary staff when any abnormalities are noted. The veterinarian can then provide medical attention and possible treatment.

22. Responsibilities of Animal Care Technicians • Animal technicians must be willing to learn as much as possible about the animals under their care and the field of laboratory animal science. • They must learn to follow instructions and perform work accurately according to prescribed standard operating procedures (SOPs). • They must report to their supervisors and principal investigators anything that could adversely affect animal welfare. For example: • Information on environmental changes • Changes in animal handling methods • Errors in the performance of day-to-day husbandry tasks. This takes knowledge, confidence, maturity and honesty.

23. Laboratory Animal Veterinarian • The laboratory animal veterinarian commonly coordinates animal care activities and advises investigators on proper animal selection and use. • In addition, the laboratory animal veterinarian is responsible for the overall maintenance of animal health and assurance of compliance with various regulations and policies that affect research animals.

24. Commercial Animal Suppliers and Equipment • Quality research depends on the laboratory equipment and supplies as well as the animals used. • Commercial animal suppliers provide animals that meet specific research requirements. Cage and specialty equipment manufacturers and feed mills must supply products that meet or exceed minimum requirements specified by current standards, laws and regulations.

25. Hygiene in the Laboratory Animal Facility • The differences among sterilization, disinfection and sanitization are a matter of degree of cleanliness. • Sterilization refers to the destruction of all organisms on an object. • Disinfection refers to reduction of the number of pathogenic microorganisms on an object to a harmless level. • Sanitization refers to reduction of the number of microorganisms on an object to an acceptable public health standard. The word "sanitization" also refers to making an object aesthetically pleasing and clean.

26. Lesson 2. Sterilization • Sterilization As stated in the previous lesson, sterilization is the destruction of all organisms on an object. Disinfection and sanitization refer to the reduction in the number of organisms, not their total destruction. Definitions for "disinfection" and "sanitization" will be reviewed in a later lesson.

27. Lesson 2. Sterilization: Autoclaving • Autoclaves sterilize items by long exposure to moisture at high temperature and pressure. Compared to other sterilization methods, autoclaving is fast, reliable and relatively cheap. Moreover, it avoids the use of toxic chemicals. • To be sterile, items should be autoclaved for at least 15 minutes at 250°F (121°C) and with a pressure of 15 pounds per square inch (psi). • The number of microorganisms that have contaminated an item and the type and size of the material being sterilized determines the autoclaving time. • For example, extra sterilization time is required for large bags of bedding, nested shoebox cages, or heavily wrapped surgical instruments.

28. Lesson 2. Sterilization: Autoclaving • How do Autoclaves work? • Autoclaves are available in types and sizes that meet a variety of needs. Most have a mechanism for regulating the steam pressure and evacuating the air inside the chamber. Like some cabinet washers, autoclaves can have clean-side and soiled-side doors. • High pressure is important. • It allows steam to be superheated, which means heated beyond its normal temperature of 212°F (100°C) at atmospheric pressure. • Superheated steam penetrates wrapped items more thoroughly for better sterilization. • Autoclaves can be dangerous if not operated properly. • Because steam under high pressure is used to achieve the temperature needed for sterilization, laboratory animal facility personnel are advised to handle autoclaves with great caution. • Animal technicians should learn all about the particular machines they are going to use before attempting to operate them.

29. Lesson 2. Sterilization: Other sterilization methods • Because of sensitivity to heat, moisture or high pressure, some items cannot be sterilized by autoclaving. Other acceptable sterilization techniques include: • gas treatment with ethylene oxide • dry heat sterilization • chemical sterilization • gamma irradiation • filtration of liquids

30. Lesson 3. Disinfection • Disinfectant Types • A disinfectant described with the suffix “cidal” is one that has killing action. • A bactericidal disinfectant kills bacteria but not necessarily spores. • A sporicidal disinfectant kills spores and bacteria. • A disinfectant that has the suffix “static” merely inhibits microorganism growth. • A bacteriostat, for example, prevents the growth of bacteria but does not necessarily kill them. • Chemicals, such as phenols, sodium hypochlorite (bleach) and quaternary ammonias, are commonly used to disinfect nonliving objects such as floors and equipment.

31. Lesson 3. Disinfection • Sodium hypochlorite • Sodium hypochlorite (bleach) is a superior disinfectant because it kills many types of bacteria and viruses. It is inexpensive and readily available. • Bleach must be used carefully, as it can harm delicate tissues such as the eyes and lungs. • Bleach does not contain dirt loosening detergents, so its effectiveness can be impaired if surfaces are not cleaned of organic matter and debris before the bleach is applied.

32. Lesson 3. Disinfection • Phenolic Compounds • At one time, phenolic compounds were popular disinfectants, even though high concentrations were needed to produce the desired level of disinfection. • It was found, however, that cats and certain other laboratory animals react adversely to phenol.

33. Lesson 3. Disinfection • Quaternary Ammonia Compounds • Quaternary ammonia compounds are weak disinfectants, even though these agents destroy the cell membranes of certain types of microorganisms. They are available as: • Virucides • Algicides • Fungicides • Quaternary ammonias are less effective when mixed with detergents or soaps because the combination neutralizes their disinfecting capability.

34. Lesson 3. Disinfection • Disinfection • Disinfection is the process by which pathogenic organisms (but not necessarily spores) are destroyed. • Disinfectants are usually too strong for use in or on living animals. • Disinfectants are sometimes classified according to the type of microorganism they are most effective against.

35. Lesson 4. Sanitization • Sanitization • Sanitization is the process by which the number of bacteria and other organisms living on inanimate objects is reduced enough to prevent disease. The process involves making the objects clean and aesthetically pleasing. • Sanitization should not be confused with disinfection, and neither term should be confused with sterilization. • Briefly: • Sanitization is a process of cleansing. • Disinfection is a process killing some microbes or preventing their growth. • Sterilization kills all microorganisms and makes the object sterile.

36. Lesson 4. Sanitization • Sanitization in an Animal Facility • In the animal facility, sanitization includes routine cleaning of items such as: • Floors • Cages • Walls • Feeders • Sinks • Implements • Tables

37. Lesson 4. Sanitization • Sanitization Process • The sanitization process involves removal of: • Dirt • Hair • Dust • Saliva • Blood • Feces • Urine • Surfaces to be sanitized are washed with detergents and rinsed with a chemical sanitizer or water at 180°F (82°C).

38. Lesson 4. Sanitization • Sanitization Procedures • Frequent sanitization of equipment and animal rooms is essential. • Chemical and microbiologic contamination must be removed by cleaning. Equipment must be monitored for substances harmful to the experimental animals. • Deodorants cannot be used in place of adequate sanitation. • In many cases, different chemicals are not compatible. The indiscriminate mixing of these chemicals can cause reactions which are hazardous to animals and people.

39. Lesson 5. Guidelines for Handling Chemicals • The following are some guidelines concerning the handling of chemicals: • Store chemicals in a cool, central area. • Follow the instructions on the label. Excess usage produces little difference in the end result and, in most cases, is hazardous • Never use a container that is not labeled. Do not make an assumption as to the contents. It is cheaper to waste something than to make a mistake. If you put a chemical in a different container you must label the new container. • Never mix two chemicals together unless authorized by the manufacturer to do so. For example, mixing ammonia and bleach produces a toxic gas. • Do not accept product samples unless they will be evaluated within a short period of time. Unused samples lying around increase the chance of misuse.

40. Lesson 6. Cage Changes • Cage Changes • According to the ILAR Guide for the Care and Use of Laboratory Animals, cages should be changed often enough "to provide a healthy environment for an animal, in accord with its normal behavior and physiologic characteristics". • Specific methods and frequencies will vary with the species and cage type, as well as other factors. • The number of animals housed in a cage, the cage size and the type of bedding are important in deciding how often to change cages.

41. Lesson 6. Cage Changes • Facilities that follow these standards generally change solid-bottomed rodent cages one to two times a week. • Most suspended drop-bottom cages are changed at least once every two weeks. • Daily cleaning and bedding replacement are essential for larger animals such as dogs, cats and nonhuman primates. • The Animal Welfare Act Regulations set minimum cleaning standards for cages, feeders and water bottles used for many laboratory animal species.

42. Lesson 7. Animal Equipment Cleaning Techniques • Cage Washers • Dirty cages and equipment should be taken to the cagewashing area for cleaning. • Trays and other equipment should not be hand cleaned in the animal room. • Hand cleaning can cause dust and microorganisms to become airborne, thus contaminating the animal room environment. • Some cagewashing areas are partitioned by walls into a clean side and a dirty side. Cagewashers used in this setting are called pass-through washers. • Soiled equipment is delivered to the door on the dirty side, where it is prepared for cleaning. • Cages and trays are scraped free of most bedding, feces and other debris.

43. Lesson 7. Animal Equipment Cleaning Techniques • Descalers • Applying chemical descalers (usually an acid solution) in the cages removes most of the urine scale. Appropriate protective equipment (gloves and goggles) should be worn when using these acid solutions.

44. Lesson 7. Animal Equipment Cleaning Techniques • Transferring Clean Cages to Storage • Cages are then put into the cagewasher to remove any remaining debris or chemicals and to sanitize them. Following sanitation in the cagewasher, the equipment is removed through the door on the clean side. From there it is transferred to storage areas or returned to use. • Nonpartitioned cagewashing areas should also have designated clean and dirty areas to help prevent contamination of clean equipment.

45. Lesson 7. Animal Equipment Cleaning Techniques • Cleaning Cage Accessories • Feeders and Dishes • After properly disposing of uneaten food, feeders and dishes are scrubbed free of debris. • They may be soaked in disinfectant before being put through the cagewasher. • The proper soaking time is usually listed on the label of the disinfectant container.

46. Lesson 7. Animal Equipment Cleaning Techniques • Water Bottles • A bottle brush may be needed to loosen deposits inside watering bottles. • Emptied bottles are placed mouth down on the holding racks in a bottle washing machine. • After manual rinsing, sipper tubes are usually washed in a cage or tunnel washer. • Bottles are refilled either one at a time under a standard faucet, or by means of an automatic bottle filler, which fills a rack of bottles at a time.

47. Lesson 7. Animal Equipment Cleaning Techniques • Cleaning Large Equipment • Equipment too large to fit into cagewashers should be vigorously hand-scrubbed or pressure washed, using detergents and disinfectants, then rinsed with water before use. • Feed and bedding containers should be cleaned and disinfected before refilling. They should be dried thoroughly after cleaning, as moisture can promote microbial growth and spoilage.

48. Lesson 8. Animal Room Cleaning Schedules and Techniques • Animal Room Cleaning Schedules • How often animal rooms are cleaned depends on the type of animals housed and the animal facility’s cleaning program. • Sinks should be clean, free of clutter, and stocked with soap and towels. • Vents and doors should be kept free of dust, grime, hair, and feathers. • Clogged vents reduce air circulation. • Trash cans should have disposable plastic liners and should be emptied frequently. They should be disinfected regularly.

49. Lesson 8. Animal Room Cleaning Schedules and Techniques • Animal Room Cleaning • Animal rooms, storage rooms and corridors should be cleaned with appropriate detergents and disinfectants as often as needed to keep them free of dirt and contaminants. • Animal rooms should be emptied, cleaned and disinfected on a fixed schedule depending on the species housed. • Cleaning should include walls, ceilings, lights and all other exposed surfaces. • Sinks, brooms, mops and buckets in animal rooms should be cleaned after each use. Mop heads should be replaced or laundered frequently. • Cleaning items (mops, buckets, brooms) should remain in the animal room where they are used, and be used ONLY for the cleaning of that room. This reduces the chance of spreading disease from room to room.

50. Lesson 9. Other Equipment Changes • Caging Accessories • Regular sanitization of racks, feeders, watering devices and other pieces of equipment keeps them clean and contamination-free. • Racks with built-in cages, for example, should be washed at least twice monthly. • Cleaning schedules for feeders depend on the number and type of animals being fed and the type of diet. • It is critical that water bottles, automatic water valves and sipper tubes be inspected daily to be sure they are working properly. • Replacing used water bottles with sanitized and full bottles ensures that the animals have all the clean water they need. • To prevent cross-contamination when bottles are refilled rather than replaced, they must be put back on the cages from which they came. • Replacing water vessels once or twice a week is usually an acceptable routine.