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Mendel’s Contribution(s)?

Mendel’s Contribution(s)?. This Week. Finish C. elegans experiment, Tinker with wasps, Exam Review (April 20 th ) Exam I (April 23 rd ), Basic Mendel! Basic Mendel! Basic Mendel! Basic Mendel! Basic Mendel! C. elegans ! Bioinfomatics! Nasonia!. Mendel’s First Postulate.

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Mendel’s Contribution(s)?

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  1. Mendel’s Contribution(s)?

  2. This Week • Finish C. elegans experiment, • Tinker with wasps, • Exam Review (April 20th) • Exam I (April 23rd), • Basic Mendel! • Basic Mendel! • Basic Mendel! • Basic Mendel! • Basic Mendel! • C. elegans! • Bioinfomatics! • Nasonia!

  3. Mendel’s First Postulate Unit Factors in Pairs • Genetic characteristics are controlled by unit factors that exist in pairs* in individual organisms, • each individual receives one unit factor from each parent*, • in a monohybrid cross, three combinations of unit factors are possible, * not in Nasonia males!

  4. Mendel’s Second Postulate Dominant/Recessive • When two unlike unit factors are present in a single individual, one unit factor is dominant to the other, which is said to be recessive.

  5. Mendel’s Third Postulate Segregation • During the processes of heredity, the paired unit factors separate so that the offspring receives one unit factor from each parent, • The unit factors segregate to offspring randomly.

  6. Mendel’s Forth Postulate Independent Assortment • How do two traits segregate in the offspring of an individual that is heterozygous for both traits?

  7. Independent Assortment…checking complementation in the F2 P: F1: F2: AAdd x aaDD AaDd 9: A_D_ 3: aaD_ 3: A_dd 1: aadd P: F1: F2: aadd x AADD AaDd 9: A_D_ 3: aaD_ 3: A_dd 1: aadd Both Recessive? Both Dominant? One of each? 9:7 wt : mut 1:15 wt : mut 3:13 wt : mut

  8. P to F1Haplo/Diplo: Thinking about complementation groups… Both recessive: aaDD x Ad  female: AaDd, male aD Both Dominant: aaDD x Ad  female: AaDd, male aD One dominant*: aadd x AD  female: AaDd, male ad or… AADD x ad  female: AaDd, male AD * need to define which is dominant!

  9. P to F1 to “F2”Haplo/Diplo: Thinking about complementation groups… Both recessive: aaDD x Ad  female: AaDd  ¼AD + ¼Ad + ¼aD + ¼ad Both Dominant: aaDD x Ad  female: AaDd  ¼AD + ¼Ad + ¼aD + ¼ad One dominant*: aadd x AD  female: AaDd  ¼AD + ¼Ad + ¼aD + ¼ad or… AADD x ad  female: AaDd  ¼AD + ¼Ad + ¼aD + ¼ad * need to define which is dominant!

  10. 1/2 x 1/2 = 1/4 AD ½ D ½ d 1/2 x 1/2 = 1/4 Ad 1/2 A 1/2 D 1/2 x 1/2 = 1/4 aD ½ d 1/2 x 1/2 = 1/4 ad 1/2 a Forked-Line MethodHaplo/Diplo F2AaDd x AD* (*male genotype could be ad, aD, Ad) 1 AD = ¼ AADD 1 AD = ¼ AaDD 1 AD = ¼ AADd 1 AD = ¼ AaDd

  11. Forked-Line MethodHaplo/Diplo F2AaDd x AD* (*male genotype could be ad, aD, Ad) x 1 AD = ¼ AADD x 1 ad = ¼ AaDd 1/4 AD 1/4 AD 1/4 AD 1/4 AD 1/4 Ad 1/4 Ad 1/4 Ad 1/4 Ad x 1 AD = ¼ AADd x 1 ad = ¼ Aadd 1/4 aD 1/4 aD 1/4 aD 1/4 aD x 1 AD = ¼ AaDD x 1 ad = ¼ aaDd x 1 AD = ¼ AaDd x 1 ad = ¼ aadd 1/4 ad 1/4 ad 1/4 ad 1/4 ad Have Fun! x 1 Ad = ¼ AADd x 1 aD = ¼ AaDD x 1 Ad = ¼ AAdd x 1 aD = ¼ AaDd x 1 Ad = ¼ aaDd x 1 aD = ¼ aaDD x 1 Ad = ¼ Aadd x 1 aD = ¼ aaDd

  12. LabLife Cycles

  13. Wasp Development TimelinesLife Cycles, Mating, etc.

  14. C. elegans Nomenclature Tower of Babel

  15. This Week • Wednesday, • score F3s, • discuss Mendel Review Formal Lab Write-Up, • think about or tinker with wasps? • Friday, • midterm, • think about or tinker with wasps?

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