1 / 13

Novel Protein design

Novel Protein design. BTY516: protein engineering . Question. Why design novel proteins? what can be gained by the design and characterization of novel proteins? Can we design 3D protein structures that have never been observed?

stormy
Télécharger la présentation

Novel Protein design

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Novel Protein design BTY516: protein engineering

  2. Question • Why design novel proteins? • what can be gained by the design and characterization of novel proteins? • Can we design 3D protein structures that have never been observed? • Are the 3D structures that have not been observed in nature unattainable, or just unsampled?

  3. Main Motivations • our understanding of natural proteins--their folding pathways, thermodynamic stabilities, and catalytic properties -- is enhanced (and ultimately tested) by our ability to design novel proteins with predetermined structures and properties. • For solving important chemical or biochemical problems.

  4. STRATEGIES FOR THE DESIGN OF STRUCTURE • Self-Assembly of Modular Units of Secondary Structure End-on view of two parallel (α-helices forming a coiled-coil structure. A single heptad repeat is shown and the view is looking from the N-terminal end with the a-helices descending into the page. Schematic diagram showing the self-assembly of two peptides into an ordered dimeric structure.

  5. STRATEGIES FOR THE DESIGN OF STRUCTURE Schematic illustration of the incremental approach

  6. STRATEGIES FOR THE DESIGN OF STRUCTURE 2- Ligand-lnduced Assembly Ligand-induced assembly of a three-helix bundle.

  7. STRATEGIES FOR THE DESIGN OF STRUCTURE 2- Ligand-lnduced Assembly Schematic diagram showing ligand-induced assembly.

  8. STRATEGIES FOR THE DESIGN OF STRUCTURE 3- Assembly of Peptides via Covalent Cross-linking Schematic diagram showing the assembly of a structure that is stabilized by covalent cross-link

  9. STRATEGIES FOR THE DESIGN OF STRUCTURE The amino acid sequence and cross-linking pattern of Chymohelizyme

  10. STRATEGIES FOR THE DESIGN OF STRUCTURE 4- Assembly of Peptides on a Synthetic Template Schematic diagram of a template-assembled synthetic protein (TASP).

  11. STRATEGIES FOR THE DESIGN OF STRUCTURE 5-Linear Polypeptides That Fold into Globular Structures Ribbon drawing of the sequence and proposed structure of Felix. The Cysll-Cys71 disulfide bond is indicated

  12. Reference Carey, P.R. (1996). Protein Engineering and Design. Academic Press.

More Related