Inhibition of Nrf2 Activity by Dominant-Negative Nrf2 Vector in Colo-357 Cells

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This study investigates the impact of a dominant-negative Nrf2 (DN-Nrf2) vector on Nrf2 activity in Colo-357 cells. Following a 24-hour transfection with either the control or DN-Nrf2 vector, cells were harvested, and total cell lysates were analyzed through immunoblotting. The results confirmed the inhibition of Nrf2 activity by measuring target gene products, including HO-1. This approach provides insight into the role of Nrf2 signaling in cellular responses and its potential therapeutic implications in cancer biology.

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May 05, 2026

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Inhibition of Nrf2 Activity by Dominant-Negative Nrf2 Vector in Colo-357 Cells

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Supplementary 1 AsPC-1 Colo-357 Control DN-Nrf2 - - + + - - + + Nrf2 GCLC HO-1 b-actin Supplementary 1.Inhibition of Nrf2 activity by DN-Nrf2 vector was confirmed by measuring Nrf2 target gene products (e.g., HO-1). After 24 hr of transfection with control or DN-Nrf2 vector, cells were harvested, and total cell lysates were used for immunoblotting assay analysis as described in Material and Methods.

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