'Cell lysates' diaporamas de présentation

PURIFICATION AND CHARACTERIZATION OF PROTEINS

PURIFICATION AND CHARACTERIZATION OF PROTEINS. BY ROHAN MENON. INTRODUCTION. Goals of purification vary with the intended use of the protein.

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Carol M. Artlett 1 , Sihem Sassi-Gaha 1 , Mitali Purohit 1 , Richard F. Rest 1 , and James D. Thacker 1,2

An endogenous lipopeptide activates the inflammasome promoting the clearance of Methicillin-Resistant Staphylococcus aureus (MRSA) Skin and Soft Tissue Infections. Carol M. Artlett 1 , Sihem Sassi-Gaha 1 , Mitali Purohit 1 , Richard F. Rest 1 , and James D. Thacker 1,2

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Vector Control

A. B. C. PELP1-shRNA. control. PELP1. PELP1shRNA. Vector Control. Vector Control. PELP1shRNA. Actin. Con-shRNA. ZR-75. MCF-7. PELP1. E. D. Actin. *. % Survival. *. *. *. PELP1-shRNA. % Survival. *. *. IOMM-LEE cells. 293T cells. Mitomycin C. Mitomycin C.

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Protein standard Whole cell lysate Cytosolic fraction Ni + -NTA affinity column

A. B. 120. 1. 2. 3. 4. 5. 6. prhTRAL. prhTRAL. c rhTRAL. c rhTRAL. 100. 80. Protein standard Whole cell lysate Cytosolic fraction Ni + -NTA affinity column Polymixin B Sepharose column PD-10 desalting column . 60. Cell viability (%). 40. 20. 0. 0. 50. 100. 150.

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Supplement Figure 5

(XB130). (XB130). Supplement Figure 5. A. B. C.

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vector BRaf wt V599E D593V G463R G565R G465E

A B C. BRaf. vector BRaf wt V599E D593V G463R G565R G465E. cell lysates. pospho-Erk. BRaf. vector Braf wt V599E D593V G463R G465R G465E. kinase assay. pospho-Erk.

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PARP

A. B. Antagomir Control miR-130b. Dex Treatment - + - +. GR. PARP. GAPDH.

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TrxR

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