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Analysis of Renilla Activity per mg Protein and RNA Copy Number in UTR Constructs

This study investigates Renilla luciferase activity in relation to total protein concentrations and RNA copy numbers using various UTR constructs: p5’UTRTat1-Renilla, p5’UTRTat2-Renilla, and p5'UTRg-Renilla. Activity measurements are reported in relation to total protein (mg) and RNA copy numbers at multiple concentrations of pTat across different constructs. The results aim to elucidate the activity profiles of Renilla luciferase under different experimental conditions, offering insights into RNA and protein interactions.

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Analysis of Renilla Activity per mg Protein and RNA Copy Number in UTR Constructs

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  1. A Renilla activity per mg total proteins B Renilla activity per RNA copy number p5’ UTRTat1-Renilla 104 10 p5’ UTRTat1-Renilla 8 Renilla activity per mg protein 0,06 6 0,05 4 0,04 2 0,03 0 Renilla activity/ RNA copy number 0 2 4 8 12 20 (ng pTat) 0,02 0,01 p5’ UTRTat2-Renilla 0 0 20 40 80 120 200 (ng pTat) 104 10 8 Renilla activity per mg protein 6 4 2 0 p5’UTRg-Renilla 0 2 4 8 12 20 (ng pTat) 0,03 0,025 p5’ UTRg-Renilla 0,02 Renilla activity/ RNA copy number 104 0,015 10 0,01 8 0,005 6 Renilla activity per mg protein 0 4 0 20 40 80 120 200 (ng pTat) 2 0 0 2 4 8 12 20 (ng pTat)

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