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SEQUENCING ITS REGIONS OF rDNA USING PRIMERS 18S & 26S KN58 & KNRV

SEQUENCING ITS REGIONS OF rDNA USING PRIMERS 18S & 26S KN58 & KNRV. 18S TTGATTACGTCCCTGCCCTTT 26S TTTCACTCGCCGTTACTAAGG Vrain et al. 1992 KN58 GTATGTTTGGTTGAGGGTC KNRV CACGCTCATACAACTGCTC.

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SEQUENCING ITS REGIONS OF rDNA USING PRIMERS 18S & 26S KN58 & KNRV

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  1. SEQUENCINGITS REGIONS OF rDNAUSINGPRIMERS 18S & 26S KN58 & KNRV

  2. 18S TTGATTACGTCCCTGCCCTTT26STTTCACTCGCCGTTACTAAGGVrain et al. 1992KN58GTATGTTTGGTTGAGGGTCKNRVCACGCTCATACAACTGCTC

  3. SEQUENCES OF 10 SPECIESS. bicornutum18S - GTA….1016 bp….TTT - 26SS. carpocapsae18S - GTA…...987 bp….TTT - 26SS. feltiae18S - GTA…...980 bp….TTT - 26SS. glaseri18S - GTA…...988 bp….TTT - 26SS. intermedium18S - GTA…..1061 bp....TTT - 26SS. monticolum18S - GTA…….916 bp...TTT - 26SS.neocurtillae18S - GTA….....968 bp…TTT- 26SS. oregonense18S - GTA …....973 bp…TTT- 26SS. scapterisci18S - GTA…….959 bp....TTT- 26SS. sp18S - GTA…. .1022 bp…TTT- 26S

  4. LENGTH OF ITS15.8SITS2S. bicornutum 281 157 329S. carpocapsae279 157 301S. feltiae275 157 298S. glaseri279 157 302S. intermedium302(-142) 157 349(-34)S. monticolum264 157 244(-139)S.neocurtillae257 157 304S. oregonense267 157 297S. scapterisci247(-217) 157 307S. sp301 158 313C. elegans464 154 383

  5. Composition Species A% C% G% T% AT% S. bicornutum 26 17 23 34 60 S. carpocapsae24 17 23 35 59 S. feltiae 26 18 23 33 58 S. glaseri24 222727 51 S. intermedium 25 17 22 36 61 S. monticolum 27 17 24 32 59 S. neocurtillae28 152037 65 S. oregonense 25 19 23 32 57 S. scapterisci 25 17 23 35 61 S. sp. 25 21 25 29 54

  6. SIMILARITY RATIOS L S. feltiae 849 S.oregonense 980 S. carpocapsae 558 S. scapterisci 572 S. glaseri 1130 S. sp. 1000 S. intermedium 671 S. glaseri 1130 Similarity 90% 89% 80% 58%

  7. S. feltiae 90% S. oregonense S. glaseri 58% S. intermedium

  8. S. bicornutum 769µm S. feltiae 100 849µm S. oregonense 980µm S. sp. 75 1000 µm 100 S. glaseri 1130µm S. carpocapsae 85 100 558µm S. scapterisci 66 572 µm 100 S.neocurtillae 885µm S. monticolum 706µm S. intermedium 671µm

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