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GUS mRNA Accumulation in Arabidopsis: Comparison Between Wild Type and sde1-1 Background

This study investigates the accumulation of GUS mRNA in Arabidopsis, comparing the wild type (Wt) and the sde1-1 background transformed with various GUS transgene constructs. Total RNA was isolated from 9-10 randomly selected T1 transformants with single-copy T-DNA insertions for each construct. The GUS gene fragment was probed to analyze transcript levels and sizes. A control sample using RNA from T1 transformants with the pGts construct in the wild-type background is included for comparison. Ethidium bromide staining of rRNA is shown for loading assessment.

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GUS mRNA Accumulation in Arabidopsis: Comparison Between Wild Type and sde1-1 Background

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  1. sde1-1 sde1-1 sde1-1 sde1-1 sde1-1 sde1-1 sde1-1 Wt Wt Wt Wt Wt Wt Wt 1 1 1 1 1 1 1 C C C C C C C 6 6 6 6 6 6 6 2 2 2 2 2 2 2 3 3 3 3 3 3 3 4 4 4 4 4 4 4 5 5 5 5 5 5 5 7 7 7 7 7 7 7 10 10 10 10 10 10 10 1 1 1 1 1 1 1 6 6 6 6 6 6 6 2 2 2 2 2 2 2 8 8 8 8 8 8 8 9 9 9 9 9 9 9 3 3 3 3 3 3 3 4 4 4 4 4 4 4 5 5 5 5 5 5 5 7 7 7 7 7 7 7 8 8 8 8 8 8 8 9 9 9 9 9 9 9 -6.0 -6.0 -6.0 -6.0 -2.0 -2.0 -2.0 -2.0 kb pGts rRNA pGtn rRNA pGtsGGts rRNA pGtstn rRNA -6.0 pGGGts -2.0 rRNA -6.0 -2.0 pG rRNA -6.0 -2.0 pGECts rRNA

  2. Supplemental Figure 2. GUS mRNA Accumulation in Arabidopsis Wild Type (Wt) and sde1-1 Background Transformed with various GUS transgene constructs. Total RNA was isolated from 9-10 randomly selected T1 transformants containing single copy T-DNA insertion for each construct in the wild-type (Wt) or sde1-1 background and probed with the GUS gene fragment. For comparison of the size and level of GUS transcripts, total RNA pooled from T1 transformants harboring the pGts construct in the wild-type background was included in the RNA blots as a control (C). The ethidium bromide stain of rRNA is shown for each lane to allow assessment of equal loading.

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