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pGECt s

sde1-1. sde1-1. sde1-1. sde1-1. sde1-1. sde1-1. sde1-1. Wt. Wt. Wt. Wt. Wt. Wt. Wt. 1. 1. 1. 1. 1. 1. 1. C. C. C. C. C. C. C. 6. 6. 6. 6. 6. 6. 6. 2. 2. 2. 2. 2. 2. 2. 3. 3. 3. 3. 3. 3. 3. 4. 4. 4. 4. 4. 4. 4. 5. 5. 5. 5. 5. 5. 5. 7.

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pGECt s

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  1. sde1-1 sde1-1 sde1-1 sde1-1 sde1-1 sde1-1 sde1-1 Wt Wt Wt Wt Wt Wt Wt 1 1 1 1 1 1 1 C C C C C C C 6 6 6 6 6 6 6 2 2 2 2 2 2 2 3 3 3 3 3 3 3 4 4 4 4 4 4 4 5 5 5 5 5 5 5 7 7 7 7 7 7 7 10 10 10 10 10 10 10 1 1 1 1 1 1 1 6 6 6 6 6 6 6 2 2 2 2 2 2 2 8 8 8 8 8 8 8 9 9 9 9 9 9 9 3 3 3 3 3 3 3 4 4 4 4 4 4 4 5 5 5 5 5 5 5 7 7 7 7 7 7 7 8 8 8 8 8 8 8 9 9 9 9 9 9 9 -6.0 -6.0 -6.0 -6.0 -2.0 -2.0 -2.0 -2.0 kb pGts rRNA pGtn rRNA pGtsGGts rRNA pGtstn rRNA -6.0 pGGGts -2.0 rRNA -6.0 -2.0 pG rRNA -6.0 -2.0 pGECts rRNA

  2. Supplemental Figure 2. GUS mRNA Accumulation in Arabidopsis Wild Type (Wt) and sde1-1 Background Transformed with various GUS transgene constructs. Total RNA was isolated from 9-10 randomly selected T1 transformants containing single copy T-DNA insertion for each construct in the wild-type (Wt) or sde1-1 background and probed with the GUS gene fragment. For comparison of the size and level of GUS transcripts, total RNA pooled from T1 transformants harboring the pGts construct in the wild-type background was included in the RNA blots as a control (C). The ethidium bromide stain of rRNA is shown for each lane to allow assessment of equal loading.

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