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Jeopardy!. DNA. Protein Synthesis. Regulation. 1 00. 1 00. 1 00. 2 00. 2 00. 2 00. 3 00. 3 00. 3 00. 4 00. 4 00. 4 00. 5 00. 5 00. 5 00. Final Jeopardy!. DNA for 100. What are the two reasons why adenine matches up with thymine in a base pair ? They form two hydrogen bonds

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  1. Jeopardy! DNA Protein Synthesis Regulation 100 100 100 200 200 200 300 300 300 400 400 400 500 500 500 Final Jeopardy!

  2. DNA for 100 • What are the two reasons why adenine matches up with thymine in a base pair? • They form two hydrogen bonds • The base pair length is consistent with G-C

  3. DNA for 200 • What is the function of topoisomerase? • Breaks and reforms bonds upstream of the replication fork to enable easier enzyme function at the fork.

  4. DNA for 300 • What are the two limitations of DNA polymerase? • Cannot start a new strand on its own. • Can only bind to the 3’ end of a strand.

  5. DNA for 400 • Why is the new strand always shorter than the template strand in DNA replication? • The final primer on the lagging strand cannot be replaced.

  6. DNA for 500 • Why do eukaryotic cells replicate DNA at an overall faster rate than prokaryotes? • Multiple origins of replication on eukaryotic chromosomes.

  7. Protein Synthesis for 100 • Daily Double! • What’s your wager? A polypeptide is being produced on the rough ER. What do you know about the protein that will result? It will become a permanent part of a membrane or will be secreted from the cell.

  8. Protein Synthesis for 200 • What is a promoter, and how can it affect gene expression? • It’s the point of attachment of RNA polymerase. Its activity level can vary, so this determines how frequently the gene will be transcribed.

  9. Protein Synthesis for 300 • What are two purposes of post-transcriptional modification in eukaryotic cells? • Splicing enables cells to make multiple proteins from the same gene. • Cap and tail prolong RNA life, enable movement through nuclear pores, and allow ribosomal attachment.

  10. Protein Synthesis for 400 • Contrast silent and missense mutations. • Silent = no impact on the polypeptide. • Missense = one or more AAs are different.

  11. Protein Synthesis for 500 • 1st 500 • 2nd 400 • 3rd 300 • 4th 200 • 5th 100 • 6th 000 Transcribe the following template strand…

  12. Regulation for 100 • Name two cellular controls that can increase the likelihood of gene expression. • Uncoiling DNA into euchromatin • Histone acetylation

  13. Regulation for 200 • In a repressible operon, what is the default state of the repressor protein, and how can this be changed? • Inactive • Binding to the product

  14. Regulation for 300 • How can a proto-oncogene become an oncogene? • Mutation • Amplification • Transposition

  15. Regulation for 400 • How does a cell recycle useless proteins? • Proteasomes break them apart into amino acids

  16. Regulation for 500 • What advantage does the blocking of translation provide for a cell? • Savings of time, energy, and raw materials. • mRNA’s build up for rapid translation later.

  17. FINAL JEOPARDY 5’ - A A G C U A U G C G A U UU A G C G A C U G A U C A G C C U A - 3’ Methionine Arginine Phenylalanine Serine Aspartate

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