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Chemical Exploration of Walnut ( Juglans regia )

Chemical Exploration of Walnut ( Juglans regia ). Jawameer Hama. Samples. 12 types of kernel of walnut, five of kernels are shelled. 5 types of walnut oil. Extraction of Oil with n- Heptane.

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Chemical Exploration of Walnut ( Juglans regia )

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  1. Chemical Exploration of Walnut (Juglansregia) Jawameer Hama

  2. Samples • 12 types of kernel of walnut, five of kernels are shelled. • 5 types of walnut oil

  3. Extraction of Oil with n-Heptane • Flaked kernels of walnut (10 g) mixed with n-Heptane (50 ml x2) in a flask, stirred for 3 hours. • The solvent is filtered, then dried by (MgSO4). • Finally n-Heptane was removed by rotary evaporator,the product was golden yellow oil.

  4. Result Relative percentage % of oil, moisture and ash content

  5. Profile of Walnut Oil

  6. Trans-esterification of Oil + Glycerol Transesterification of a triacylglycerol to its fatty acids butyl ester (FABE). Palmitic acid butyl ester

  7. GC-MS data • GC was used to analyse the FABE, which had been produced by trans-esterification. • In GC-TOF-MS, the column was OmegaWax. Linoleic acid butyl ester (C18:2) Palmitic acid butyl ester (C16:0)

  8. GC-MS Fatty acid butyl ester assignment and relative percentage (%) of individual fatty acids in walnut oil extracted from Kurdish walnuts by GC- TOF- MS

  9. 1H-NMR of Crude Walnut Oil Glycerol

  10. 13C- NMR of Crude Walnut Oil C18 Terminal carbon Olefinic region C16 C1 Carbonyl Glycerol

  11. 13C- NMR of Olefinic Region Linoleic acid C18:2 Eicosenoic acid C20:1 ∆11 Vaccenic acid C18:1 ∆11 Oleic acid C18:1 ∆9 Linolenic acid C18:3

  12. 13C- NMR of Crude Walnut Oil C18:2 C18:3 C18:1 ∆ 9 C16:0 C18:1 ∆ 11 C20:1 C18:0 C16:1

  13. Total Phenolic Content in Defatted Kernel Folin-Ciocalteu reagent was used to find out total phenolic content. Gallic acid was used as standard.

  14. Total Phenolic Content (mg GAE/g) of Defatted Kernels of Walnut

  15. Conclusions • More than half the weight of walnut kernels (41-64 %) is oil. • NMR analysis can be more precise and quicker for fatty acid analysis. • Defatted kernel contains high level of total phenolic content.

  16. Acknowledgments • Kurdistan Region Government • Dr. Vera Thoss • Dr. Dave Preskett • Toby Vye • Thank you for listening

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