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Aim of my study

Oxidative stress and neonatal health CH Lin PC Chen Institute of Occupational Medicine and Industrial Hygiene, College of Public Health, National Taiwan University. Aim of my study

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Aim of my study

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  1. Oxidative stress and neonatal healthCH Lin PC ChenInstitute of Occupational Medicine and Industrial Hygiene,College of Public Health, National Taiwan University Aim of my study My study is focused on the possible effects of oxidative stress, generated from various environmental exposures, on the birth outcome. Background Oxidative stress(OS) occurs while the balance between reactive oxygen species(ROS) and antioxidants being disrupted towards an overabundance of ROS. Being a double-edged sword, ROS serves not only as key signal molecules in physiological processes but also play a role in pathological processes involving the female reproductive tract. It affects multiple physiological processes from oocyte maturation to fertilization, embryo development and pregnancy. ROS has also been found to be more or less related to endometriosis and pathophysiology of infertility and assisted fertility, preeclampsia, hydatidiform mole, , preterm labour, gestational diabetes, free radical-induced birth defects and other situations such as abortions. Pregnancy itself is a state of oxidative stress. The ROS produced in a normal pregnancy have pronounced effects on placental function including trophoblast proliferation and differentiation and vascular reactivity. However, OS, being defined by the evidence of either an increase in lipid peroxides and isoprostanes or a decrease in expression and activity of antioxidants, has be found been associated with several pathological processes, including early pregnancy loss in first trimester, gestational diabetes, IUGR, and preeclampsia in late gestation. Materials and Methods A total of 486 pairs of mother and neonate were recruited in Taiwan Birth Panel Study in one medical center, one local hospital, and two clinics in Taipei area, Taiwan, from May 2004 to January 2005. The questionnaire was used to collect the basic information, maternal health status, and maternal exposure, and the maternal and cord blood and placenta were also collected and stored at -80℃ at birth. Another questionnaire involved two inventories, CDIIT and HOME, was completed in 6 months’ and 24 months’ follow-up in order to determine neonatal health status, the developmental status and the environmental determinants. Heavy metals, including Pb, As, Hg, Cd, and Mn, cotinine in maternal and/or cord blood were analyzed. Markers of maternal infections of cytomegalovirus(CMV) and toxoplasmosis, using both their IgG and IgM antibodies were measured. Immunoglobulin E antibody was analyed. 8OHdG is thought to be a useful biomarker for oxidative stress and is analyzed by UPLC/MS/MS. SNPs of Four metabolic genes, CYP1A1 MspI, CYP1A1 Ile462Val, GSTT1 and GSTM1 were identified. Works to do DNA was extracted in 180 samples and 8-OHdG was analyzed. However, more than 90% of the samples were under detection limits. We are now considering some modification in the methods and will further analyze if possible.

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