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Biological Membranes

This document explores the applications of neutron and X-ray scattering techniques in the study of biological membranes, focusing on lipid bilayers, phase behavior, and structural properties. It discusses various aspects such as membrane composition, the role of cholesterol, and the effects of osmotic pressure and thermal stress. Additionally, the significance of lipid mixtures, phase separation, and the impact of antimicrobial peptides on membrane rigidity are examined, providing insights into the complexities of biological membrane dynamics and structure.

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Biological Membranes

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  1. Biological Membranes Penghui Lin Nov. 15. 2013

  2. Membranes http://www.uvm.edu/~inquiryb/webquest/fa06/mvogenbe/

  3. Diffusion across plasma membrane http://en.wikipedia.org/wiki/Cell_membrane

  4. Plasma Membrane Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.ChemPhys Lipids. 2010 Jun;163(6):460-79

  5. Man-made membrane http://bioweb.wku.edu/courses/biol115/wyatt/biochem/lipid/Lipid_2.asp

  6. Liquid crystals • Nematic phase • Smecticphases • Chiral phases http://en.wikipedia.org/wiki/Liquid_crystal

  7. Sample preparation Jörg Fitter, Thomas Gutberlet, John Katsaras. Neutron Scattering in Biology, Techniques and Applications. 2006ISBN: 978-3-540-29108-4 (Print) 978-3-540-29111-4 (Online)

  8. Specular Neutron Reflectivity Incident angle = reflected angle Jörg Fitter, Thomas Gutberlet, John Katsaras. Neutron Scattering in Biology, Techniques and Applications. 2006ISBN: 978-3-540-29108-4 (Print) 978-3-540-29111-4 (Online)

  9. Density profile ρ0(z) Fourier Synthesis Jörg Fitter, Thomas Gutberlet, John Katsaras. Neutron Scattering in Biology, Techniques and Applications. 2006ISBN: 978-3-540-29108-4 (Print) 978-3-540-29111-4 (Online)

  10. Lipid bilayer phases

  11. Location of Cholesterol Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.ChemPhys Lipids. 2010 Jun;163(6):460-79

  12. Location of cholesterol Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.ChemPhys Lipids. 2010 Jun;163(6):460-79

  13. Forces between membranes Osmotic pressure Disjoining pressure Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.ChemPhys Lipids. 2010 Jun;163(6):460-79

  14. Osmotic stress experiment Neutron/ Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.ChemPhys Lipids. 2010 Jun;163(6):460-79

  15. Thermal unbinding Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.ChemPhys Lipids. 2010 Jun;163(6):460-79

  16. Single component membrane Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.ChemPhys Lipids. 2010 Jun;163(6):460-79

  17. Lipid mixtures and domains • Ideal mixing: no phase separation • Non-ideal or non-random mixing: compositional fluctuation happens while no stable domains formed • Macroscopic phase separation: micron-size stable lipid domains are formed

  18. No phase separation Membrane thickness Fluctuation parameter in DMPC bilayers at 35° Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.ChemPhys Lipids. 2010 Jun;163(6):460-79

  19. Macroscopic phase separation DPPC/DOPC/cholesterol Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.ChemPhys Lipids. 2010 Jun;163(6):460-79

  20. Supramolecular properties Tilted phase Interdigitated phase Diffused scattering Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.ChemPhys Lipids. 2010 Jun;163(6):460-79

  21. Bicelle mixtures Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.ChemPhys Lipids. 2010 Jun;163(6):460-79

  22. Bicelle mixtures Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.ChemPhys Lipids. 2010 Jun;163(6):460-79

  23. Fluctuation change upon antimicrobial peptide binding Jörg Fitter, Thomas Gutberlet, John Katsaras. Neutron Scattering in Biology, Techniques and Applications. 2006ISBN: 978-3-540-29108-4 (Print) 978-3-540-29111-4 (Online)

  24. Membrane rigidity change Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.ChemPhys Lipids. 2010 Jun;163(6):460-79

  25. Pabst G. Applications of neutron and X-ray scattering to the study of biologically relevant model membranes J.ChemPhys Lipids. 2010 Jun;163(6):460-79

  26. Thanks! Jörg Fitter, Thomas Gutberlet, John Katsaras. Neutron Scattering in Biology, Techniques and Applications. 2006ISBN: 978-3-540-29108-4 (Print) 978-3-540-29111-4 (Online)

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