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This document presents a comprehensive analysis of RT-PCR reactions grouped by various parameters such as molecular weight (MW) and transfection units (Tr.Un.G). The study is divided into four groups focusing on different PDI (Protein Disulfide Isomerase) levels, providing insights into cloning vectors and their mapping. Each group's results detail specific reactions, their efficiencies, and implications for cloning studies. This analysis aims to facilitate better understanding and optimization of RT-PCR methodologies in genetic research.
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RT-PCR ReactionsGroup 1 (PDI 1) MW Tr Un G - MW Tr Un G - MW Tr Un G - MW Tr Un G -
RT-PCR ReactionsGroup 2 (PDI 1) MW Tr Un G - MW Tr Un G - MW Tr Un G -
RT-PCR ReactionsGroup 3 (PDI 2) MW Un Tr G - MW - Tr Un G MW Un Tr G -
RT-PCR ReactionsGroup 4 (PDI 2) Un Tr G - MW Un Tr G - MW