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Fluorescent principle

Fluorescent principle. Fluorescent microscope. DAPI. FITC. Alexa 488. Tetramethylrhodamine/TRITC. Rhodamin. Rhodamin Red-X. Alexa 568. Alexa 647. Texas-Red and Texas-Red-X. Alexa 594. Cy-Series. GFP and GFPuv. E-GFP's and DsRed. Table of Fluorescent Proteins.

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Fluorescent principle

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  1. Fluorescent principle

  2. Fluorescent microscope

  3. DAPI

  4. FITC

  5. Alexa 488

  6. Tetramethylrhodamine/TRITC

  7. Rhodamin

  8. Rhodamin Red-X

  9. Alexa 568

  10. Alexa 647

  11. Texas-Red and Texas-Red-X

  12. Alexa 594

  13. Cy-Series

  14. GFP and GFPuv

  15. E-GFP's and DsRed

  16. Table of Fluorescent Proteins

  17. GFP is a 238-amino acid protein originally isolated from the bioluminescent jellyfish Aequoria victoria Can be expressed in any organism without additional cofactors What is green fluorescent protein (GFP) ?

  18. Structure of GFP • Structurally, GFP represents a new motif which has been named the “β-can” • The “can” consists of 11 β-strands and a central α-helix

  19. Fluorescence is mediated by a fluorophore located at the center of the protein GFP-Fluorophore

  20. Structure of the GFP-fluorophore • The fluorophore is derived from the tripeptide -Ser65-Tyr66-Gly67- • Formation of the fluorophore occurs in an autocatalytic manner

  21. Light sources for Fluorescence

  22. Filtersetsand GFP's

  23. Quantum efficiency of Chip

  24. New Filterset

  25. New Filterset-other flourochromes

  26. Old Filtersets

  27. Old filtersets- other flurochromes

  28. Antibodies used for fluorescence

  29. Laser and Fluorochromes

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