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High-Throughput Kinase Screening and Imaging of Selected siRNA Subset with Control Validation

This study presents a comprehensive analysis of kinase activity utilizing microscopy images from a high-throughput screening (HTS) of a selected siRNA subset. The experiment comprises three plates with two replicates each, evaluating the effects of eight controls per plate, including Rluc and various kinases (KIF11, KIF23, CLSPN, CASP1, PLK1, TRAPPC3, UBC, CD3EAP). The quality of the screens is assessed through image-based calculations, offering insights into kinase function and cellular responses.

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High-Throughput Kinase Screening and Imaging of Selected siRNA Subset with Control Validation

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Presentation Transcript

  1. Kinase screen • Microscopy images • Kinase siRNA subset • 3 plates, 2 replicates • 8 controls per plate: • Rluc • KIF11, KIF23, CLSPN, CASP1, PLK1, TRAPPC3, UBC CD3EAP

  2. imageHTS webQuery

  3. imageHTS cellPicker

  4. Screen quality

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