Laboratory demonstration - DC and T cell activation

Jul 18, 2012

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Basic parametersApplicationsCell phenotypingMeasurement of cell activationVisualization of cell divisionDetection of intracellular molecues (e.g. cytokine)Analysis of specific ligand bindingAnalysis of intracellular calcium concentrationAnalysis of DNA content Cell sorting. Applications o

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Laboratory demonstration - DC and T cell activation

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1. Applications of flow cytometry in basic immunology Generation and characterization of DC Assays for T cell activation Cell proliferation Cell division Cell activation marker Laboratory demonstration - DC and T cell activation

2. Basic parameters Applications Cell phenotyping Measurement of cell activation Visualization of cell division Detection of intracellular molecues (e.g. cytokine) Analysis of specific ligand binding Analysis of intracellular calcium concentration Analysis of DNA content Cell sorting Applications of Flow Cytometry in Basic Immunology

3. FSC (forward scatter) - Size SSC (side scatter) - Granularity FL (flurochrome) � FL-1, FL-2, FL-3 etc. Flow cytometry - the basic parameters

4. (1) FSC � the size

T Cell Maturation and Activation

T Cell Maturation and Activation February 15, 2001 Outline T Cell Maturation Early development in the thymus Positive Selection and Negative Selection CD4 + T Cells vs. CD8 + T Cells T Cell Activation Signal Transduction/Gene Expression Costimulation Expansion vs. Anergy

914 views • 22 slides

Laboratory demonstration - DC and T cell activation

Laboratory demonstration - DC and T cell activation. Applications of flow cytometry in basic immunology Generation and characterization of DC Assays for T cell activation Cell proliferation Cell division Cell activation marker. Applications of Flow Cytometry in Basic Immunology.

933 views • 32 slides

Modeling T-Cell Activation Using Visual Formalism

Outline. IntroductionWhat is this Biological System?StatechartsModelResults and Conclusion. Introduction. From analysis (finding building blocks)To synthesis (integration of parts)requires language of math;sinceThe definition of reactive systems suits biological systems at different levels

424 views • 29 slides

T Cell Activation

T Cell Activation. Costimulation, Inhibition, and the Immunologic Synapse. Immunology’s “Dirty Little Secret”. Early experiments demonstrated that antigen-derived (i.e. TCR-mediated) signals alone are insufficient to initiate an immune response

884 views • 29 slides

B-Cell Generation, Activation, and Differentiation

B-Cell Generation, Activation, and Differentiation. B-Cell Maturation B-Cell Activation and Proliferation The Humoral Response In Vivo Sites for Induction of Humoral Responses Germinal Centers and Antigen-Induced B-Cell Differentiation Regulation of the Immune Effector Response.

2.32k views • 35 slides

B CELL DEVELOPMENT AND ACTIVATION

B CELL DEVELOPMENT AND ACTIVATION. In healthy people, there are mature B cells with the capacity to make antibodies to virtually any antigen. Bone marrow is the primary lymphoid organ in which B cell development occurs. .

1.42k views • 86 slides

T cell Maturation, Activation & Differentation

T cell Maturation, Activation & Differentation. Hugh B. Fackrell. T cell Maturation, Activation & Differentation. Assigned Reading Content Outline Performance Ojectives Key terms Key Concepts Short Answer Questions. Assigned Reading. Chapter: 12 pp 285-310

410 views • 13 slides

Chapter 10 T-Cell Maturation, Activation, and

Chapter 10 T-Cell Maturation, Activation, and Differentiation. 本章大綱 : T-Cell Maturation and the Thymus Thymic Selection of the T-Cell Repertoire T H -Cell Activation T-Cell Differentiation Cell Death and T-Cell populations Peripheral gd T-Cells.

1.02k views • 52 slides

ANTIGEN-SPECIFIC T – CELL ACTIVATION PARTNERS

ANTIGEN-SPECIFIC T – CELL ACTIVATION PARTNERS Antigen presenting cell carrying antigenic peptides bound to MHC – pre-formed MHC – peptide complexes Self MHC with captured antigenic peptide interacts with antigen-specific T lymphocytes selected from the available T cell repertoire INTERACTION

348 views • 16 slides

The Two-Signal Model of T-cell Activation

The Two-Signal Model of T-cell Activation. DC. T cell. CD4 or CD8. 1+2=Full activation. 1. TCR. MHC. 2. COSTIMULATION. Experimental evidences for the two signal model of T cell activation Experimental evidences that CD28 is a costimulatory receptor

568 views • 28 slides

T-cell activation positively correlates with cell-associated

T-cell activation positively correlates with cell-associated HIV-DNA level in PBMCs in viremic patients with acute or chronic HIV-1 infection.

114 views • 1 slides

B Cell Generation, Activation, And Differentiation

B Cell Generation, Activation, And Differentiation. W. Robert Fleischmann, Ph.D. Department of Urologic Surgery University of Minnesota Medical School rfleisch@umn.edu (612) 626-5034. Objectives. To understand B cell development

1.26k views • 31 slides

Chapter 10 T-Cell Maturation, Activation, and Differentiation

Chapter 10 T-Cell Maturation, Activation, and Differentiation. MHC. CD4/CD8. TCR. zz. gde. Dec 7 & 12, 2006. 你需要學習的課題： 1. 什麼叫做 positive selection 及 negative selection ？它們產生什麼結果？ 2. T 細胞需要 costimulatory signals 才得完全活化。 3. 什麼叫做 clonal anergy ？

1.2k views • 43 slides

T-Cell Maturation, Activation, And Differentiation

T-Cell Maturation, Activation, And Differentiation. W. Robert Fleischmann, Ph.D. Department of Urologic Surgery University of Minnesota Medical School rfleisch@umn.edu (612) 626-5034. Objectives. To understand T cell maturation in the thymus, including positive and negative selection

846 views • 35 slides

T CELL BIOLOGY T cell development T cell activation T cell responses

Lecture Blocks. T CELL BIOLOGY T cell development T cell activation T cell responses. SPECIAL CELLS, PLACES AND THINGS NK Cells, Mucosa, Cytokines. IMMUNE REGULATION Regulation of Immunity Infections and vaccines Autoimmunity/Tolerance Immunology of Pregnancy. Course Material.

859 views • 28 slides

T-Cell Maturation, Activation, and Differentiation

T-Cell Maturation, Activation, and Differentiation. T-Cell Maturation and the Thymus Thymic Selection of the T-Cell Repertoire TH-Cell Activation T-Cell Differentiation Cell Death and T-Cell Populations. 1.T-Cell Maturation and the Thymus.

2.08k views • 17 slides

B – CELL ACTIVATION

B – CELL ACTIVATION. Ligand. Ligand. SIGNAL. SIGNAL. RECEPTOR MEDIATED CELL ACTIVATION. Cross - linking. C onformational change. CROSS – LINKING OF THE RECEPTOR INITIATES A SIGNALING CASCADE. ligand. kinase activation. phosphorylation. r ecruitment o f adaptors.

624 views • 33 slides

Molecules involved in T cell activation

Ce ll-mediated im mune response Activation of T lymphocytes by intracellular microorganisms Phases of T cell response Biochemical pathways of T cell activation Functional responses of T lymphocytes to antigens and costimulation. Molecules involved in T cell activation. TCR. TCR complex.

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ANTIGEN PRESENTATION T – CELL RECOGNITION T – CELL ACTIVATION T – CELL EFFECTOR FUNCTIONS

ANTIGEN PRESENTATION T – CELL RECOGNITION T – CELL ACTIVATION T – CELL EFFECTOR FUNCTIONS. CLP. T CELLS. B CELLS. Common lymphoid precursor. T. B. Th. CTL. PC. Activate B cells and macrophages T HELPER CELLS. Kill virus- infected cells CYTOTOXIC T LYMPHOCYTES.

483 views • 22 slides

Chapter 10- T-cell maturation, Activation, & Differentiation

Chapter 10- T-cell maturation, Activation, & Differentiation. Where we’re going- Define these words Positive and negative selection Some terms for the steps along the way to maturity Be in awe of how complex activation is (not really- hard to test!), and understand the bottom line.

521 views • 33 slides

T cell activation assay

Antigen specific T cell activation assay is one of the technologies we can provide during the one-stop service which can activate CAR-T cells for further downstream research or clinical trials. T cell activation requires at least two signals to be fully activated. The first signal is provided by the interaction of T cell antigen-specific receptor and the antigen-major histocompatibility complex (MHC). https://www.creative-biolabs.com/car-t/antigen-specific-t-cell-activation-assay.htm

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V-set domain containing T cell activation inhibitor 1

B7H4 belongs to the B7 family (see CD80; MIM 112203) of costimulatory proteins. These proteins are expressed on the surface of antigen-presenting cells and interact with ligands (e.g., CD28; MIM 186760) on T lymphocytes.

42 views • 4 slides

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Laboratory demonstration - DC and T cell activation

Laboratory demonstration - DC and T cell activation

Presentation Transcript

T Cell Maturation and Activation

Laboratory demonstration - DC and T cell activation

Modeling T-Cell Activation Using Visual Formalism

T Cell Activation

B-Cell Generation, Activation, and Differentiation

B CELL DEVELOPMENT AND ACTIVATION

T cell Maturation, Activation &amp; Differentation

Chapter 10 T-Cell Maturation, Activation, and

ANTIGEN-SPECIFIC T – CELL ACTIVATION PARTNERS

The Two-Signal Model of T-cell Activation

T-cell activation positively correlates with cell-associated

B Cell Generation, Activation, And Differentiation

Chapter 10 T-Cell Maturation, Activation, and Differentiation

T-Cell Maturation, Activation, And Differentiation

T CELL BIOLOGY T cell development T cell activation T cell responses

T-Cell Maturation, Activation, and Differentiation

B – CELL ACTIVATION

Molecules involved in T cell activation

ANTIGEN PRESENTATION T – CELL RECOGNITION T – CELL ACTIVATION T – CELL EFFECTOR FUNCTIONS

Chapter 10- T-cell maturation, Activation, &amp; Differentiation

T cell activation assay

V-set domain containing T cell activation inhibitor 1

T cell Maturation, Activation & Differentation

Chapter 10- T-cell maturation, Activation, & Differentiation