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This supplemental figure presents a detailed examination of the generation of CrGFP-expressing UVM11 transgenic lines. It includes a schematic diagram of the pCrGFP construct (part A), confirmed integration of the construct into the transgenic genome through Southern blot analysis (part B), and Northern blot analysis demonstrating CrGFP expression levels in the transgenic lines, monitored by methylene blue staining (part C). Additionally, part D illustrates the detection of CrCFP accumulation via western blot, providing insights into the expression dynamics of the transgenic constructs.
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Supplemental Fig. 1 a pCrGFP rbcS2 P β2-Tub P CrGFP psaD T rbcS2 T aph7” Intron 1 rbcS2 b UVM11 1 2 3 4 5 6 7 8 9 10 11 12 c UVM11 1 2 3 4 5 7 8 9 10 11 12 CrGFP d UVM11 1 2 3 4 5 7 8 9 CrGFP ~27 KD Supplementary Figure 1. Generation of CrGFP-expressing UVM11 transgenic lines. A. Schematic diagram of a pCrGFP construct. B. Integration of the construct in the genome of transgenic line, confirmed by Southern blot. C. Northern blot analysis to determine CrGFP expression in transgenic lines. RNA amount was monitored by methylene blue staining D. Detection of CrCFP accumulation by western blot.
