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This study investigates the role of P311 in adenovirus infection and stable transfection using a GFP plasmid control. The relative P311 levels were analyzed post-infection and transfection, showcasing changes in GFP expression and localization within C2C12 cells. Results demonstrate significant alterations in fluorescence levels, with a focus on the P311-adenovirus interplay. The study aims to elucidate the molecular mechanisms underlying P311's involvement in these processes, offering insights into potential therapeutic targets for modulating viral infections and stable transfections.
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A. P311-adenovirus infection P311-stable transfection 4.0 P311 0.14 P311 3.5 GFP Plasmid control 0.12 3.0 0.10 2.5 Relative P311 level 0.08 2.0 0.06 1.5 0.04 1.0 0.02 0.5 0 0 3I 3I 3D 3I 6D PM DM 3D DM 6D P311-adenovirus GFP control adenovirus GFP positive cells 70m FLAG localisation GFP C2C12 only P311 P311-adenovirus infection P311-stable transfection 600000 600000 500000 500000 400000 400000 Plasmid control P311 C2C12 only fluorescence (560/590nm)/ mg protein 300000 300000 200000 200000 100000 100000 0 0 3I 3I 3D 3I 6D 3I 10D DM 10D PM DM 3D DM 6D B. C.