biocontrol of bacterial seedling rot and grain rot of rice n.
Skip this Video
Loading SlideShow in 5 Seconds..
Biocontrol of “Bacterial Seedling Rot and Grain Rot of Rice” PowerPoint Presentation
Download Presentation
Biocontrol of “Bacterial Seedling Rot and Grain Rot of Rice”

Biocontrol of “Bacterial Seedling Rot and Grain Rot of Rice”

263 Vues Download Presentation
Télécharger la présentation

Biocontrol of “Bacterial Seedling Rot and Grain Rot of Rice”

- - - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript

  1. Biocontrol of “Bacterial Seedling Rot and Grain Rot of Rice” Causal Agent: Burkholderia glumae

  2. Introduction:Problem to be addressed • To identify antibacterial activity of organic and aqueous extracts from Skeletonemacostatum(Diatom)against Burkholderia glumae, which is a causal Agent of “Bacterial Seedling Rot and Grain Rot of Rice” in the in vitro conditions. • One of the most destructive diseases of rice.

  3. The Causal agent: Burkholderia Glumae • A gram-negative soil bacteria. • Formerly named Pseudomonas glumae. • Causal agent of grain rot and seedling rot of rice. • Burkholderia glumae produces Toxoflavin, which is toxic for plant. • This Toxoflavin generate superoxide & peroxide, which causes, Bacterial Seedling Rot and Grain Rot of Rice.

  4. Symptoms of Disease • Infection is symptomized by brown, water-soaked soft rot of the rice. • Wilting or soft rot of the leaves sheath. • Spikelet sterility with poor or no grain ripening. • Leads to the death of the plant.

  5. Biological Treatment & its Necessity • Use of chemicals & antimicrobial agents in paddy field may harm the normal soil flora. • Toxic for human health. • Thus, a biological treatment for the pathogen applied in the paddy field, can be worthy & harmless.

  6. Treatment • Skeletonemacostatum (Bacillariophyceae) a marine, filamentous, planktonic diatom, common in Indian coastal water, produces Quinolone like compounds. • Thus an attempt has been made to use Skeletonemacostatumfor the inhibition of the bacteria of interest.

  7. The Objective • Use of Skeletonemacostatum as an antagonist for the bacteria of interest. • The aim is to find new biological agents for control of the disease, remaining plant unaffected.

  8. Experiment • Isolation of the bacteria from plant- A selective medium - CCNT • 2 g of yeast extract • 1 g of polypepton • 4 g of inositol • 10 mg of cetrimide • 10 mg of chloramphenicol • 1 mg of novobiocin • 100 mg of chlorotharonil • 18 g of agar in 1 liter of distilled water (adjusted to pH 4.8 )

  9. Incubation of B. glumae. • Formation of yellowish white colony with a diffusible yellow pigment. • Other microorganisms were detected at a much lower rate on the CCNT medium. • For organic and aqueous extracts of Skeletonemacostatum – MASM (Modified Artificial Seawater Medium) along with Acetone and methanol in distilled water is used.

  10. Agar Ditch Method • The isolated & screened Burkholderiaglumaefrom CCNT medium is sub-cultured on agar media • Incubation • A ditch was made in the plate with the help of a sterile cup-borer (0.85 cm)

  11. The organic and aqueous extracts of Skeletonemacostatumwere introduced aseptically and the petri-plates were incubated at 270C (+10C) for 3-4 days. • Determination of the Antibacterial activity and microbial growth.

  12. Observation and Result • Test organism inhibition zone- 15.0mm (+ 3.21). • This shows a high antibacterial activity of Skeletonema costatum against Burkholderia glumae.

  13. Conclusion • Conditions which would enhance the antibacterial agent production from algae should be studied for the biological control of the Seedling Rot and Grain Rot of Rice due to Burkholderiaglumae. • To make it practicable in natural conditions of crop growth.

  14. Applications and future prospects • This in vitro studied can be applied with some modification to prevent the diseases in paddy fields. • Development of the transgenic plants with improved qualities. • For designing novel compounds for agriculture. • Can be long lasting, inexpensive and harmless to the life. • Biocontrol systems eliminate neither pathogen nor diseases but bring them into natural balance.

  15. Thankyou!!!