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Amino Acids, Peptides and Proteins

Amino Acids, Peptides and Proteins. (continued). Box 1 fig 1. Fig 5-6. Fig 5-16. table 5-2. Table 5-3. Fig 5-17. Fig 5-18. Fig 5-18b. Fig 5-18c. HPLC. Most often for analytical (as opposed to preparative) purposes Separation Principles Same as column chromatography (above)

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Amino Acids, Peptides and Proteins

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  1. Amino Acids, Peptides and Proteins (continued)

  2. Box 1 fig 1

  3. Fig 5-6

  4. Fig 5-16

  5. table 5-2

  6. Table 5-3

  7. Fig 5-17

  8. Fig 5-18

  9. Fig 5-18b

  10. Fig 5-18c

  11. HPLC • Most often for analytical (as opposed to preparative) purposes • Separation Principles • Same as column chromatography (above) • Also types specific to LC • HIC • Reverse Phase

  12. Electrophoresis • Both analytical and preparative uses • Gel • Column • Gel bed • Microcolumn (capillary) • Free Solution CE • MEKC

  13. Electrophoresis • Mobility   = V/E = Z/f

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