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This study utilizes DIGE technology to compare depleted plasma samples labeled with distinct fluorescent tags, eliminating gel-to-gel variations. An internal standard is run on all gels for cross-gel sample comparisons.
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Sample 1 – Cy3 Sample 2 – Cy5 Overlay Image Samples are labelled with different fluorescent tags, allowing 2 samples to be separated on a single gel, removing gel-to-gel variation. An internal standard, composed of all samples in the experiment, is run on each gel, allowing comparison of samples on different gels. DIGE comparison of depleted plasma