1 / 9

Gel Interpretation & DNA Clean-up

In the Spring 2010 Tucson High School Biotechnology course, students conducted DNA gel electrophoresis experiments to analyze various virus DNA samples, including psbA, DNA polymerase, and g23. Students explored the significance of positive and negative controls to determine expected results. The focus was on interpreting gel results to confirm hypotheses about myoviruses and podoviruses. Further, discussions on DNA clean-up processes, including PCR reaction components and methodologies, highlighted the importance of maintaining sample integrity for sequencing.

umay
Télécharger la présentation

Gel Interpretation & DNA Clean-up

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Gel Interpretation&DNA Clean-up Tucson High School Biotechnology Course Spring 2010

  2. What have we done so far? negative control psbA virus DNA positive control negative control DNA pol virus DNA positive control negative control g23 virus DNA positive control

  3. What samples should we see a band for? negative control NO psbA MAYBE virus DNA positive control YES negative control NO DNA pol virus DNA MAYBE positive control YES negative control NO g23 virus DNA MAYBE positive control YES

  4. Interpreting your gel psbA DNApol g23 Negative control Positive control Virus Negative control Positive control Virus Negative control Positive control Virus

  5. What should you write about your gel?

  6. Hypothesis Myovirus Podovirus X X psbA X DNA pol X g23

  7. Next… Sequencing Need to Clean Up DNA PCR reaction taq polymerase dNTPs primers MgCl2 buffer DNA clean up DNA

  8. MinElutepcr Clean-Up pcr products Buffer PE Buffer EB KEEP!! MinElute Column Buffer PB MinElute Column MinElute Column

  9. Which pcr products will you clean up? psbA DNApol g23 Negative control Positive control Virus Negative control Positive control Virus Negative control Positive control Virus

More Related