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Dead-End Metabolites

Dead-End Metabolites. by Markus Krummenacker Q4 2011. Dead-End Metabolites. Knowledge of cellular metabolism is incomplete, so some metabolites can be dead-ends in the reaction network. Either only consumed, or only produced by their reactions

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Dead-End Metabolites

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  1. Dead-End Metabolites by Markus Krummenacker Q4 2011

  2. Dead-End Metabolites • Knowledge of cellular metabolism is incomplete, so some metabolites can be dead-ends in the reaction network. • Either only consumed, or only produced by their reactions • Affects whether some reactions in FBA can carry flux. Dead-ends can block flux. • EcoCyc Example: spermidine biosynthesis. S-methyl-5-thio-D-ribose had to be listed in the secretions set.

  3. Dead-End Metabolite Finder • Command under Tools menu • Choice whether to ignore reactions of unknown direction, or to treat them as reversible • Resulting dead-end metabolites are shown on Cellular Overview • Report file written to *cyc/version/reports/

  4. Remedies • More curation: create missing metabolic or transport reactions • Shortcut: Add to secretions (or nutrients) set in .fba file • In MILP mode, the new keyword all-compounds can used for the try-secretions parameter. The try-secretion-weight should be set to a negative value, or else too many compounds are added.

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