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FSS-i 3

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FSS-i 3

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  1. FSS-i3 DNA expert system suite

  2. DNA Profiling • Sample e.g. hair, skin

  3. DNA Profiling • Sample e.g. hair, skin • Process sample to obtain raw data

  4. DNA Profiling • Sample e.g. hair, skin • Process sample to obtain raw data • Produce a profile of the DNA

  5. DNA Profiling • Sample e.g. hair, skin • Process sample to obtain raw data • Produce a profile of the DNA • Store profile in NDIS (National DNA Index System) or match to existing profile

  6. Expert System profiling motivation • Sample & data processing already heavily automated

  7. Expert System profiling motivation • Sample & data processing already heavily automated • Sample data analysis; DNA profiling is the bottle neck.

  8. Expert System profiling motivation • Sample & data processing already heavily automated • Sample data analysis; DNA profiling is the bottle neck. • Two independent data reviews required to upload a profile to NDIS

  9. Expert System profiling motivation • Sample & data processing already heavily automated • Sample data analysis; DNA profiling is the bottle neck. • Two independent data reviews required to upload a profile to NDIS • Save enormous amounts of review time

  10. FSS-i3 Expert System • FSS: Forensic Science Service of the UK

  11. FSS-i3 Expert System • FSS: Forensic Science Service of the UK • Developed a software suit called FSS-i3 • 6 years of R&D and validation

  12. FSS-i3 Expert System • FSS: Forensic Science Service of the UK • Developed a software suit called FSS-i3 • 6 years of R&D and validation • Designed by forensic scientists for forensic scientists

  13. FSS-i3 Expert System • FSS: Forensic Science Service of the UK • Developed a software suit called FSS-i3 • 6 years of R&D and validation • Designed by forensic scientists for forensic scientists • Made available to forensic community • USA, Canada, Europe, Australia, New Zealand

  14. FSS-i3 Expert System • FSS: Forensic Science Service of the UK • Developed a software suit called FSS-i3 • 6 years of R&D and validation • Designed by forensic scientists for forensic scientists • Made available to forensic community • USA, Canada, Europe, Australia, New Zealand • Full Audit system

  15. i-STRess • The core DNA interpretation tool

  16. i-STRess • The core DNA interpretation tool • Produces single source DNA profiles using configurable rules & filters

  17. i-STRess • The core DNA interpretation tool • Produces single source DNA profiles using configurable rules & filters • Detect multi-person DNA mixtures within a profile & export to i-STReam…

  18. i-STReam • Unique functionality to resolve two person DNA mixtures

  19. i-STReam • Unique functionality to resolve two person DNA mixtures • Allows previously unusable DNA profiles to be uploaded to DNA databases

  20. i-ntegrity • DNA samples/data can become contaminated: accidental DNA mixture

  21. i-ntegrity • DNA samples/data can become contaminated: accidental DNA mixture • System flags possible profile contaminations for further review

  22. i-ntegrity • DNA samples/data can become contaminated: accidental DNA mixture • System flags possible profile contaminations for further review • Saves time: no more manual reviews of profiles

  23. FSS-i3 Validation • FSS-i3 developed from internal FSS expert systems • 6 years of testing

  24. FSS-i3 Validation • FSS-i3 developed from internal FSS expert systems • 6 years of testing • 1 million+ profiles uploaded to UK National DNA DB

  25. FSS-i3 Validation • FSS-i3 developed from internal FSS expert systems • 6 years of testing • 1 million+ profiles uploaded to UK National DNA DB • FSS-i3 validation project: • 70 000 previously analyzed samples • Minor discrepancies in 0.03% of samples & each of these samples were flagged by the system for further review

  26. Technology used in FSS-i3 • Strategies for implementing rules: unknown

  27. Technology used in FSS-i3 • Strategies for implementing rules: unknown • User optimization: 20+ rules settings

  28. Technology used in FSS-i3 • Strategies for implementing rules: unknown • User optimization: 20+ rules settings • Designed to run on stand-alone Pentium 3 PC’s

  29. Design & Development of FSS-i3 • Development time: six years

  30. Design & Development of FSS-i3 • Development time: six years • “The FSS developers who designed the FSS-i3 suite are, first and foremost, DNA scientists who understand the problems forensic scientists face” (1)

  31. Design & Development of FSS-i3 • Development time: six years • “The FSS developers who designed the FSS-i3 suite are, first and foremost, DNA scientists who understand the problems forensic scientists face” (1) • “i-STReam is based on scientific theory developed by Dr Peter Gill and Dr Tim Clayton” (1)

  32. Conclusions: FSS-i3 • Consistent profiling of samples

  33. Conclusions: FSS-i3 • Consistent profiling of samples • Ability to interpret mixed DNA profiles

  34. Conclusions: FSS-i3 • Consistent profiling of samples • Ability to interpret mixed DNA profiles • Check for contamination

  35. Conclusions: FSS-i3 • Consistent profiling of samples • Ability to interpret mixed DNA profiles • Check for contamination • Significant reduction in time spent on the above, due to automation

  36. References • (1) http://www.forensic.gov.uk/forensic_t/i3/index.htm • (2) http://www.promega.com/profiles/802/profilesindna_802_08.pdf • (3) http://forensics.marshall.edu/NEST/Nest%20PDFs/Documents/NIJEvalExpertSys.pdf • (4) http://www.promega.com/profiles/1001/profilesindna_1001_11.pdf • (5) http://www.kathyreichs.com/dnaprofiling.htm • (6) http://www.biotechnologyonline.gov.au/human/dnaprofile.cfm