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FSS-i 3

FSS-i 3. DNA expert system suite. DNA Profiling. Sample e.g. hair, skin. DNA Profiling. Sample e.g. hair, skin Process sample to obtain raw data. DNA Profiling. Sample e.g. hair, skin Process sample to obtain raw data Produce a profile of the DNA. DNA Profiling. Sample e.g. hair, skin

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FSS-i 3

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  1. FSS-i3 DNA expert system suite

  2. DNA Profiling • Sample e.g. hair, skin

  3. DNA Profiling • Sample e.g. hair, skin • Process sample to obtain raw data

  4. DNA Profiling • Sample e.g. hair, skin • Process sample to obtain raw data • Produce a profile of the DNA

  5. DNA Profiling • Sample e.g. hair, skin • Process sample to obtain raw data • Produce a profile of the DNA • Store profile in NDIS (National DNA Index System) or match to existing profile

  6. Expert System profiling motivation • Sample & data processing already heavily automated

  7. Expert System profiling motivation • Sample & data processing already heavily automated • Sample data analysis; DNA profiling is the bottle neck.

  8. Expert System profiling motivation • Sample & data processing already heavily automated • Sample data analysis; DNA profiling is the bottle neck. • Two independent data reviews required to upload a profile to NDIS

  9. Expert System profiling motivation • Sample & data processing already heavily automated • Sample data analysis; DNA profiling is the bottle neck. • Two independent data reviews required to upload a profile to NDIS • Save enormous amounts of review time

  10. FSS-i3 Expert System • FSS: Forensic Science Service of the UK

  11. FSS-i3 Expert System • FSS: Forensic Science Service of the UK • Developed a software suit called FSS-i3 • 6 years of R&D and validation

  12. FSS-i3 Expert System • FSS: Forensic Science Service of the UK • Developed a software suit called FSS-i3 • 6 years of R&D and validation • Designed by forensic scientists for forensic scientists

  13. FSS-i3 Expert System • FSS: Forensic Science Service of the UK • Developed a software suit called FSS-i3 • 6 years of R&D and validation • Designed by forensic scientists for forensic scientists • Made available to forensic community • USA, Canada, Europe, Australia, New Zealand

  14. FSS-i3 Expert System • FSS: Forensic Science Service of the UK • Developed a software suit called FSS-i3 • 6 years of R&D and validation • Designed by forensic scientists for forensic scientists • Made available to forensic community • USA, Canada, Europe, Australia, New Zealand • Full Audit system

  15. i-STRess • The core DNA interpretation tool

  16. i-STRess • The core DNA interpretation tool • Produces single source DNA profiles using configurable rules & filters

  17. i-STRess • The core DNA interpretation tool • Produces single source DNA profiles using configurable rules & filters • Detect multi-person DNA mixtures within a profile & export to i-STReam…

  18. i-STReam • Unique functionality to resolve two person DNA mixtures

  19. i-STReam • Unique functionality to resolve two person DNA mixtures • Allows previously unusable DNA profiles to be uploaded to DNA databases

  20. i-ntegrity • DNA samples/data can become contaminated: accidental DNA mixture

  21. i-ntegrity • DNA samples/data can become contaminated: accidental DNA mixture • System flags possible profile contaminations for further review

  22. i-ntegrity • DNA samples/data can become contaminated: accidental DNA mixture • System flags possible profile contaminations for further review • Saves time: no more manual reviews of profiles

  23. FSS-i3 Validation • FSS-i3 developed from internal FSS expert systems • 6 years of testing

  24. FSS-i3 Validation • FSS-i3 developed from internal FSS expert systems • 6 years of testing • 1 million+ profiles uploaded to UK National DNA DB

  25. FSS-i3 Validation • FSS-i3 developed from internal FSS expert systems • 6 years of testing • 1 million+ profiles uploaded to UK National DNA DB • FSS-i3 validation project: • 70 000 previously analyzed samples • Minor discrepancies in 0.03% of samples & each of these samples were flagged by the system for further review

  26. Technology used in FSS-i3 • Strategies for implementing rules: unknown

  27. Technology used in FSS-i3 • Strategies for implementing rules: unknown • User optimization: 20+ rules settings

  28. Technology used in FSS-i3 • Strategies for implementing rules: unknown • User optimization: 20+ rules settings • Designed to run on stand-alone Pentium 3 PC’s

  29. Design & Development of FSS-i3 • Development time: six years

  30. Design & Development of FSS-i3 • Development time: six years • “The FSS developers who designed the FSS-i3 suite are, first and foremost, DNA scientists who understand the problems forensic scientists face” (1)

  31. Design & Development of FSS-i3 • Development time: six years • “The FSS developers who designed the FSS-i3 suite are, first and foremost, DNA scientists who understand the problems forensic scientists face” (1) • “i-STReam is based on scientific theory developed by Dr Peter Gill and Dr Tim Clayton” (1)

  32. Conclusions: FSS-i3 • Consistent profiling of samples

  33. Conclusions: FSS-i3 • Consistent profiling of samples • Ability to interpret mixed DNA profiles

  34. Conclusions: FSS-i3 • Consistent profiling of samples • Ability to interpret mixed DNA profiles • Check for contamination

  35. Conclusions: FSS-i3 • Consistent profiling of samples • Ability to interpret mixed DNA profiles • Check for contamination • Significant reduction in time spent on the above, due to automation

  36. References • (1) http://www.forensic.gov.uk/forensic_t/i3/index.htm • (2) http://www.promega.com/profiles/802/profilesindna_802_08.pdf • (3) http://forensics.marshall.edu/NEST/Nest%20PDFs/Documents/NIJEvalExpertSys.pdf • (4) http://www.promega.com/profiles/1001/profilesindna_1001_11.pdf • (5) http://www.kathyreichs.com/dnaprofiling.htm • (6) http://www.biotechnologyonline.gov.au/human/dnaprofile.cfm

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