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Polymerase Chain Reaction

Polymerase Chain Reaction. PCR. “PCR is one of those inventions like the internet, once you have used it, you cannot quite understand how people managed before it existed”. Nobel Prize 1993. Invented by Dr. Kary Mullis, Research Scientist, Perkin Elmer Cetus Corporation in April 1983.

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Polymerase Chain Reaction

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  1. Polymerase Chain Reaction PCR

  2. “PCR is one of those inventions like the internet, once you have used it, you cannot quite understand how people managed before it existed”

  3. Nobel Prize 1993 • Invented by Dr. Kary Mullis, • Research Scientist, Perkin Elmer Cetus Corporation in April 1983.

  4. What is PCR? • A method of “amplifying” specific DNA sequences. • A test tube system for DNA replication that allows a “target” DNA sequence to be selectively amplified several million fold in just a few hours.

  5. How Does PCR Work? • http://www.abpischools.org.uk/resources/poster-series/pcr/pcranim.asp

  6. HOW DOES PCR WORK ?

  7. PCR Reaction: • Template DNA • Primers • dNTPs • Buffer • Taq DNA polymerase

  8. PCR Applications • Endless.

  9. Problems with PCR • To be discussed……. • Example: Bias • GC rich sequences tend to be preferentially amplified over AT rich sequences • Other sources of bias?

  10. Discussion • Paper #1: Saiki et al. (Mullis) Science • Paper #2: Bruce et al. • Presented by Mary Beth • Paper #3: Abdulmajwood et al. • Paper #4: Wang and Wang

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