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January 10, 2012

SureTag Complete DNA Labeling Kit*. Irina Sini Product Manager. * For research use only. Not for use in diagnostics procedures. January 10, 2012. CGH+SNP Workflow. SureTag Complete DNA Labeling Kit. The new kit enhances and streamlines the

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January 10, 2012

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  1. SureTag Complete DNA Labeling Kit* Irina Sini Product Manager * For research use only. Not for use in diagnostics procedures. January 10, 2012

  2. CGH+SNP Workflow Agilent Confidential

  3. SureTag Complete DNA Labeling Kit • The new kit enhances and streamlines the • workflow and contains all the components necessary for labeling: • Restriction Digestion enzymes • Labeling reagents • Clean-up columns Improved Quality of Labeling and Hybridization The labeling and hybridization performance are significantly improved compared to existing kit. Enables Complete Labeling Workflow Added Reference DNA • From single genotyped individual, both male and female, ideal as reference DNA for CGH+SNP arrays Agilent Confidential

  4. SureTag Complete DNA Labeling Kit Agilent Confidential

  5. Product Ordering Details Agilent Confidential

  6. Complete Workflow Product Bundles Each bundle contains all the consumables necessary to process a CGH and CHG+SNP microarray experiment with 48-50 customer samples Agilent Confidential

  7. Workflow Improvements • Optimized reagent formulation will enable a more robust, sensitive and accurate performance for CGH, CGH+SNP, and CNV microarray workflows • All inclusive kit with components for sample fragmentation, labeling, and purification will provide a complete labeling solution • All reagents for labeling and hybridization will be available from Agilent to simplify the ordering Agilent Confidential

  8. Workflow Improvements DNA purification Sample Fragmentation Male and Female Reference DNA, Alu I, Rsa I Restriction Digestion enzymes Random primers + 3’ Denaturation Sample Labeling Exo-Klenow Cy3 and Cy5 dyes dNTPs, Buffer Sample Purification Clean-up Columns Hybridization Cot-1 DNA Wash Scan New Components Added: Green Formulation Optimized: Blue New Labeling Kit Components: Agilent Confidential

  9. Kit Contents: Supporting a Complete Labeling Protocol Same Reagents with Improved Formulation * Agilent Confidential

  10. Reference DNA – part of the SureTag Complete DNA Labeling Kit • High Quality Product • Identified one male and one female cell lines with minimal aberrations • Stability of the genome confirmed by karyotyping, purified to produce high quality DNA • Ready to Use • DNA has been quantifyed using both Nanodrop and Qubit in the ready to use concentration of 200ng/ul • DNA has been genotyped and sequenced. A genotype file will be available on eArray, next version of the software, Cytogenomics 2.5 will have the files built in. • Stable Commercial Supply from Agilent • Have the ability to generate more of that DNA for the life of the product Agilent Confidential

  11. Cot-1 Human DNA – New Hybridization Reagent • Genomic Cot-1 Human DNA is used to block the repetitive elements to prevent non-specific hybridization. • Optimized for CGH and CGH+SNP microarray workflows. • Part of a complete CGH and CGH+SNP platform from Agilent. Agilent Confidential

  12. Competitive Positioning The Agilent SureTag Complete DNA Labeling Kit including Reference DNA and a complete set of hybridization reagents delivers the most complete solution to cytogenetics research customers providing the greatest degree of confidence in their microarray results. Agilent Confidential

  13. A Complete CGH+SNP Solution Sample Labeling Bravo Automation Catalog & Custom Microarrays Hybridization & Wash Scanning & Feature Extraction Data Analysis & Results Agilent Confidential

  14. SureTag Complete DNA Labeling KitTechnical Performance Superior technical performance • Labeling performance: • Higher yield • Better dye incorporation (specific activity) • Hybridization performance: • Higher signal • Higher signal to noise • Lower or equivalent derivative of the log ratio spread (dLRsd) Agilent Confidential

  15. SureTag DNA Labeling Kit • Superior Labeling performance: Higher yield Better dye incorporation Agilent Confidential

  16. SureTag DNA Labeling Kit High Signal High Signal to noise Low probe to probe variability • Superior Hybridization performance: Existing SureTag Agilent Confidential

  17. Functional Equivalency to the Current Kit -X Chromosome Separation Remains Highly Efficient Probe Number X Chromosomal Probes Expected log2ratio = -1 Autosomal Probes Expected log2ratio = 0 Old Kit New Kit Log2 Ratio Agilent Confidential

  18. Functional Equivalency to the Current Kit -Chromosome View: Trisomy Chr. 21 (NA04592)Catalog 4x180K CGH+SNP microarray Blue: Existing Red: SureTag 4-3 -2 -1 0.0 1.0 2.0 3.0 4.0 0 1 2 3 4 0.0 1.0 2.0 3.0 4.0 4-3 -2 -1 0 1 2 3 4 Agilent Confidential Agilent Confidential

  19. Consistent Performance between Agilent Human Reference DNA and other reference DNA samples Panel A. Agilent Reference DNA Male and NA12891 (Male HapMap) CGH+SNP microarray Panel B. Promega Male CGH microarray Agilent Confidential

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