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Surveillance of Dengue Vector Using Ovitrap

The Vectors. Aedes albopictusNative to Southeast AsiaPeri-domesticBreed and bite outdoorsAlso breed in natural containersBiting Peak : before dusk. Aedes aegyptiImported from AfricaDomesticatedBreed and bite indoorsOnly breed in artificial containersBiting peak : morning. Biology of Aedes

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Surveillance of Dengue Vector Using Ovitrap

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    1. Surveillance of Dengue Vector Using Ovitrap

    2. The Vectors Aedes albopictus Native to Southeast Asia Peri-domestic Breed and bite outdoors Also breed in natural containers Biting Peak : before dusk Aedes aegypti Imported from Africa Domesticated Breed and bite indoors Only breed in artificial containers Biting peak : morning

    3. Biology of Aedes mosquitoes in relation to dengue transmission Short breeding cycle : 7 days - 10 days Domestic breeding habitats and peri-domestic, multiple breeding sites Eggs withstand desiccation (transportation via eggs) Biting habits : day light hours Anthropophilic behaviour

    4. Biology of Aedes mosquitoes in relation to dengue transmission Vertical infection of eggs Flight range 90% less than 100m female max. ~ 500m Male max. ~ 200m Several blood meal in one single gonotropic cycle Not laying all mature eggs in one single oviposition

    5. Conventional Aedes Surveys Container Index ( proportion of +ve containers) House Index (proportion of +ve houses) Bruteau Index (relationship between +ve containers and houses)

    6. Interpretation of Transmission Risk Based on container count, not larvae The sampling unit is house or premise, hard to define in multistory apartment, blocks and public places Do not address adult productivity

    7. New Indices Pupal Index : No. of pupae / 100 houses Potential Container Index : No. of potential breeding sites / 100 houses Aedes density Index : No. of Aedes larvae / house Ovitrap Index : % of Aedes positive ovitrap

    8. Oviposition Trap Surveillance tool (airport, seaport, public areas) Assessment tool (assess specific control operation, e.g. fogging) Research tool (season prevalence, collect samples for research purposes) Control tool (autocidal ovitrap)

    9. Objectives of Ovitrap Survey To study and monitor the distribution of the Aedes albopictus at selected areas; To serve as a tool to monitor the effectiveness of mosquito prevention and control work carried out by the District Pest Control Staff of FEHD and other concerned departments; To provide surveillance information to effect changes in control strategies if necessary.

    10. Methodology Ovitrap : 200ml black plastic container 6.5 cm diameter, 10.0 cm in height Brownish oviposition paddle Fill up with water to ~ 80% full 55 Ovitraps in each survey area (~ 0.55km2 Each 100m apart

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